ArticleBiochimica et biophysica acta2009
Oleic- and docosahexaenoic acid-containing phosphatidylethanolamines differentially phase separate from sphingomyelin.
Article in Biochimica et biophysica acta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Order vs. Disorder: Cholesterol and Omega-3 Phospholipids Determine Biomembrane Organization.International journal of molecular sciences · 2022Article
- EPA and DHA differentially modulate membrane elasticity in the presence of cholesterol.Biophysical journal · 2021Article
- DHA Modifies the Size and Composition of Raftlike Domains: A Solid-StateBiophysical journal · 2018Article
- Docosahexaenoic acid lowers cardiac mitochondrial enzyme activity by replacing linoleic acid in the phospholipidome.The Journal of biological chemistry · 2018Article
- 17β-Estradiol Directly Lowers Mitochondrial Membrane Microviscosity and Improves Bioenergetic Function in Skeletal Muscle.Cell metabolism · 2018Article
- Remodelling of primary human CD4+ T cell plasma membrane order by n-3 PUFA.The British journal of nutrition · 2018Article
- Emerging role of chemoprotective agents in the dynamic shaping of plasma membrane organization.Biochimica et biophysica acta. Biomembranes · 2017Review
- Distinct membrane properties are differentially influenced by cardiolipin content and acyl chain composition in biomimetic membranes.Biochimica et biophysica acta. Biomembranes · 2017Article
- Lipid Interactions and Organization in Complex Bilayer Membranes.Biophysical journal · 2016Article
- Phase diagram of a polyunsaturated lipid mixture: Brain sphingomyelin/1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine/cholesterol.Biochimica et biophysica acta · 2016Article
- Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes.Membranes · 2015Article
- Miscibility of Sphingomyelins and Phosphatidylcholines in Unsaturated Phosphatidylcholine Bilayers.Biophysical journal · 2015Article
- Using neurolipidomics to identify phospholipid mediators of synaptic (dys)function in Alzheimer's Disease.Frontiers in physiology · 2013Article
- Docosahexaenoic and eicosapentaenoic acids segregate differently between raft and nonraft domains.Biophysical journal · 2012Article
- Biophysical and biochemical mechanisms by which dietary N-3 polyunsaturated fatty acids from fish oil disrupt membrane lipid rafts.The Journal of nutritional biochemistry · 2012Review
- The janus face of lipids in human breast cancer: how polyunsaturated Fatty acids affect tumor cell hallmarks.International journal of breast cancer · 2012Article
- Systems virology identifies a mitochondrial fatty acid oxidation enzyme, dodecenoyl coenzyme A delta isomerase, required for hepatitis C virus replication and likely pathogenesis.Journal of virology · 2011Article
- Membrane raft organization is more sensitive to disruption by (n-3) PUFA than nonraft organization in EL4 and B cells.The Journal of nutrition · 2011Article
- Docosahexaenoic acid reduces amyloid beta production via multiple pleiotropic mechanisms.The Journal of biological chemistry · 2011Article
- Dietary lipids from an evolutionary perspective: sources, structures and functions.Maternal & child nutrition · 2011Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
A central tenet of the lipid raft model is the existence of non-raft domains. In support of this view, we have established in model membranes that a phosphatidylethanolamine (PE)-containing docosahexaenoic acid (DHA) forms organizationally distinct non-raft domains in the presence of sphingomyelin (SM) and cholesterol (Chol). We have shown that formation of DHA-rich domains is driven by unfavorable molecular interactions between the rigid Chol molecule and the highly flexible DHA acyl chain. However, the molecular interactions between SM and the DHA-containing PE, which could also contribute to the formation of DHA-rich non-raft domains, have not been sufficiently investigated. To address this issue, we use differential scanning calorimetry (DSC) to study the phase behavior of mixtures of SM with either 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (16:0-22:6PE) or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (16:0-18:1PE), an oleic acid (OA)-containing control, over a wide range of concentrations. Deconvolution of binary DSC scans shows that both 16:0-22:6PE and 16:0-18:1PE phase separate from SM. Analysis of transition temperatures and partial phase diagrams, constructed from the DSC scans for the first time, shows that 16:0-22:6PE displays greater non-ideal mixing with SM compared to 16:0-18:1PE. Our findings support a model in which DHA- and OA-containing PEs differentially phase separate from SM over a wide range of molar ratios to initiate the formation of non-raft domains, which is greatly enhanced by DHA, but not OA, in the presence of cholesterol.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.