ArticleEnvironmental microbiology2010
Identification and characterization of new LuxR/LuxI-type quorum sensing systems from metagenomic libraries.
Article in Environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 69 citations in OpenAlex.
- Drugs for the Quorum Sensing Inhibition of Oral Biofilm: New Frontiers and Insights in the Treatment of Periodontitis.Pharmaceutics · 2022Review
- Review
- Functional metagenomic analysis of quorum sensing signaling in a nitrifying community.NPJ biofilms and microbiomes · 2021Article
- Thinking Outside the Bug: Molecular Targets and Strategies to Overcome Antibiotic Resistance.International journal of molecular sciences · 2019Review
- HqiA, a novel quorum-quenching enzyme which expands the AHL lactonase family.Scientific reports · 2017Article
- Functional metagenomics reveals novel β-galactosidases not predictable from gene sequences.PloS one · 2017Article
- Article
- Article
- Biotechnological applications of functional metagenomics in the food and pharmaceutical industries.Frontiers in microbiology · 2015Review
- Review
- An N-acyl homoserine lactone synthase in the ammonia-oxidizing bacterium Nitrosospira multiformis.Applied and environmental microbiology · 2014Article
- Article
- Evaluation of a pooled strategy for high-throughput sequencing of cosmid clones from metagenomic libraries.PloS one · 2014Article
- LuxR- and luxI-type quorum-sensing circuits are prevalent in members of the Populus deltoides microbiome.Applied and environmental microbiology · 2013Article
- Comparative genomic analysis of six bacteria belonging to the genus Novosphingobium: insights into marine adaptation, cell-cell signaling and bioremediation.BMC genomics · 2013Article
- Are there acyl-homoserine lactones within mammalian intestines?Journal of bacteriology · 2013Review
- A metagenomic study highlights phylogenetic proximity of quorum-quenching and xenobiotic-degrading amidases of the AS-family.PloS one · 2013Article
- Distribution and diversity of acyl homoserine lactone producing bacteria from four different soils.Current microbiology · 2013Article
- Phylogenetically novel LuxI/LuxR-type quorum sensing systems isolated using a metagenomic approach.Applied and environmental microbiology · 2012Article
- Tracking dynamics of plant biomass composting by changes in substrate structure, microbial community, and enzyme activity.Biotechnology for biofuels · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Quorum sensing (QS) cell-cell communication systems are utilized by bacteria to coordinate their behaviour according to cell density. Several different types of QS signal molecules have been identified, among which acyl-homoserine lactones (AHLs) produced by Proteobacteria have been studied to the greatest extent. Although QS has been studied extensively in cultured microorganisms, little is known about the QS systems of uncultured microorganisms and the roles of these systems in microbial communities. To extend our knowledge of QS systems and to better understand the signalling that takes place in the natural environment, metagenomic libraries constructed using DNA from activated sludge and soil were screened, using an Agrobacterium biosensor strain, for novel QS synthase genes. Three cosmids (QS6-1, QS10-1 and QS10-2) that encode the production of QS signals were identified and DNA sequence analysis revealed that all three clones encode a novel luxI family AHL synthase and a luxR family transcriptional regulator. Thin layer chromatography revealed that these LuxI homologue proteins are able to synthesize multiple AHL signals. Tandem mass spectrometry analysis revealed that LuxI(QS6-1) directs the synthesis of at least three AHLs, 3-O-C14:1 HSL, 3-O-C16:1 HSL and 3-O-C14 HSL; LuxI(QS10-1) directs the synthesis of at least 3-O-C12 HSL and 3-O-C14 HSL; while LuxI(QS10-2) directs the synthesis of at least C8 HSL and C10 HSL. Two possible new AHLs, C14:3 HSL and (?)-hydroxymethyl-3-O-C14 HSL, were also found to be synthesized by LuxI(QS6-1).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.