ArticleApplied and environmental microbiology2009
Processivity, synergism, and substrate specificity of Thermobifida fusca Cel6B.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Reduced Processivity in a Chitobiohydrolase Enhances LPMO-Assisted Chitin Depolymerization.Biochemistry · 2026Article
- There is an "I" in team: individual improvements in supercharged cellulase cocktail facilitates cooperative cellulose degradation.Biotechnology for biofuels and bioproducts · 2026Article
- Thermobifida fusca Cel6B moves bidirectionally while processively degrading cellulose.Biotechnology for biofuels and bioproducts · 2024Article
- Functional characterisation of a new halotolerant seawater active glycoside hydrolase family 6 cellobiohydrolase from a salt marsh.Scientific reports · 2024Article
- Biodegradation of agave Comiteco bagasse by3 Biotech · 2023Article
- Molecular mechanisms of processive glycoside hydrolases underline catalytic pragmatism.Biochemical Society transactions · 2023Article
- Engineering cellulases for conversion of lignocellulosic biomass.Protein engineering, design & selection : PEDS · 2023Review
- Enzyme Synergy in Transient Clusters of Endo- and Exocellulase Enables a Multilayer Mode of Processive Depolymerization of Cellulose.ACS catalysis · 2022Article
- Oxidized Product Profiles of AA9 Lytic Polysaccharide Monooxygenases Depend on the Type of Cellulose.ACS sustainable chemistry & engineering · 2021Article
- Novel Synergistic Mechanism for Lignocellulose Degradation by a Thermophilic Filamentous Fungus and a Thermophilic Actinobacterium Based on Functional Proteomics.Frontiers in microbiology · 2020Article
- Valorization of waste forest biomass toward the production of cello-oligosaccharides with potential prebiotic activity by utilizing customized enzyme cocktails.Biotechnology for biofuels · 2019Article
- Cellulases from Thermophiles Found by Metagenomics.Microorganisms · 2018Review
- Periplasmic Cytophaga hutchinsonii Endoglucanases Are Required for Use of Crystalline Cellulose as the Sole Source of Carbon and Energy.Applied and environmental microbiology · 2016Article
- Subsite-specific contributions of different aromatic residues in the active site architecture of glycoside hydrolase family 12.Scientific reports · 2015Article
- Comparison of Thermobifida fusca Cellulases Expressed in Escherichia coli and Nicotiana tabacum Indicates Advantages of the Plant System for the Expression of Bacterial Cellulases.Frontiers in plant science · 2015Article
- Kinetics of cellobiohydrolase (Cel7A) variants with lowered substrate affinity.The Journal of biological chemistry · 2014Article
- A distinct model of synergism between a processive endocellulase (TfCel9A) and an exocellulase (TfCel48A) from Thermobifida fusca.Applied and environmental microbiology · 2014Article
- Loop motions important to product expulsion in the Thermobifida fusca glycoside hydrolase family 6 cellobiohydrolase from structural and computational studies.The Journal of biological chemistry · 2013Article
- Two-parameter kinetic model based on a time-dependent activity coefficient accurately describes enzymatic cellulose digestion.Biochemistry · 2013Article
- Insights into exo- and endoglucanase activities of family 6 glycoside hydrolases from Podospora anserina.Applied and environmental microbiology · 2013Article
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2 authors.
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Abstract
A relationship between processivity and synergism has not been reported for cellulases, although both characteristics are very important for hydrolysis of insoluble substrates. Mutation of two residues located in the active site tunnel of Thermobifida fusca exocellulase Cel6B increased processivity on filter paper. Surprisingly, mixtures of the Cel6B mutant enzymes and T. fusca endocellulase Cel5A did not show increased synergism or processivity, and the mutant enzyme which had the highest processivity gave the poorest synergism. This study suggests that improving exocellulase processivity might be not an effective strategy for producing improved cellulase mixtures for biomass conversion. The inverse relationship between the activities of many of the mutant enzymes with bacterial microcrystalline cellulose and their activities with carboxymethyl cellulose indicated that there are differences in the mechanisms of hydrolysis for these substrates, supporting the possibility of engineering Cel6B to target selected substrates.
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