ArticleApplied and environmental microbiology2009
Use of inorganic and organic nitrogen by Synechococcus spp. and diatoms on the west Florida shelf as measured using stable isotope probing.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 66 citations in OpenAlex.
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- The "Dark Side" of Picocyanobacteria: Life as We Do Not Know It (Yet).Microorganisms · 2022Review
- Anoxic chlorophyll maximum enhances local organic matter remineralization and nitrogen loss in Lake Tanganyika.Nature communications · 2021Article
- Simulating metagenomic stable isotope probing datasets with MetaSIPSim.BMC bioinformatics · 2020Article
- Proteomic Response to Rising Temperature in the Marine CyanobacteriumFrontiers in microbiology · 2019Article
- SIPSim: A Modeling Toolkit to Predict Accuracy and Aid Design of DNA-SIP Experiments.Frontiers in microbiology · 2018Article
- Metagenomic Analysis of Cyanobacteria in an Oligotrophic Tropical Estuary, South Atlantic.Frontiers in microbiology · 2018Article
- Direct Utilization of Organic Nitrogen by Phytoplankton and Its Role in Nitrogen Cycling Within the Southern California Bight.Frontiers in microbiology · 2018Article
- Different Bacterial Communities Involved in Peptide Decomposition between Normoxic and Hypoxic Coastal Waters.Frontiers in microbiology · 2017Article
- A comprehensive framework for functional diversity patterns of marine chromophytic phytoplankton using rbcL phylogeny.Scientific reports · 2016Article
- Intraclade Heterogeneity in Nitrogen Utilization by Marine Prokaryotes Revealed Using Stable Isotope Probing Coupled with Tag Sequencing (Tag-SIP).Frontiers in microbiology · 2016Article
- Effects of Nitrogen Availability and Form on Phytoplankton Growth in a Eutrophied Estuary (Neuse River Estuary, NC, USA).PloS one · 2016Article
- Acclimation of Emiliania huxleyi (1516) to nutrient limitation involves precise modification of the proteome to scavenge alternative sources of N and P.Environmental microbiology · 2015Article
- Urea uptake and carbon fixation by marine pelagic bacteria and archaea during the Arctic summer and winter seasons.Applied and environmental microbiology · 2014Article
- Article
- High-sensitivity stable-isotope probing by a quantitative terminal restriction fragment length polymorphism protocol.Applied and environmental microbiology · 2012Article
- Identification of nitrogen-incorporating bacteria in petroleum-contaminated arctic soils by using [15N]DNA-based stable isotope probing and pyrosequencing.Applied and environmental microbiology · 2011Article
- Differential distributions of synechococcus subgroups across the california current system.Frontiers in microbiology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The marine nitrogen (N) cycle is a complex network of biological transformations in different N pools. The linkages among these different reservoirs are often poorly understood. Traditional methods for measuring N uptake rely on bulk community properties and cannot provide taxonomic information. (15)N-based stable isotope probing (SIP), however, is a technique that allows detection of uptake of individual N sources by specific microorganisms. In this study we used (15)N SIP methodology to assess the use of different nitrogen substrates by Synechococcus spp. and diatoms on the west Florida shelf. Seawater was incubated in the presence of (15)N-labeled ammonium, nitrate, urea, glutamic acid, and a mixture of 16 amino acids. DNA was extracted and fractionated using CsCl density gradient centrifugation. Quantitative PCR was used to quantify the amounts of Synechococcus and diatom DNA as a function of density, and (15)N tracer techniques were used to measure rates of N uptake by the microbial community. The ammonium, nitrate, urea, and dissolved primary amine uptake rates were 0.077, 0.065, 0.013, and 0.055 micromol N liter(-1) h(-1), respectively. SIP data indicated that diatoms and Synechococcus spp. actively incorporated N from [(15)N]nitrate, [(15)N]ammonium, and [(15)N]urea. Synechococcus also incorporated nitrogen from [(15)N]glutamate and (15)N-amino acids, but no evidence indicating uptake of labeled amino acids by diatoms was detected. These data suggest that N flow in communities containing Synechococcus spp. and diatoms has more plasticity than the new-versus-recycled production paradigm suggests and that these phytoplankters should not be viewed strictly as recycled and new producers, respectively.
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