ArticleJournal of bacteriology2010
Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 51 citations in OpenAlex.
- An anaerobic pathogen rewires host metabolism to fuel oxidative growth in the inflamed gut.Cell · 2026Article
- Activation and modulation of the host response to DNA damage by an integrative and conjugative element.Journal of bacteriology · 2025Article
- Transcription termination and antitermination are critical for the fitness and function of the integrative and conjugative element Tn916.PLoS genetics · 2024Article
- Activation and modulation of the host response to DNA damage by an integrative and conjugative element.bioRxiv : the preprint server for biology · 2024Article
- Characterization of ConE, the VirB4 Homolog of the Integrative and Conjugative Element ICEJournal of bacteriology · 2023Article
- Timing of integration into the chromosome is critical for the fitness of an integrative and conjugative element and its bacterial host.PLoS genetics · 2023Article
- TnSmu1 is a functional integrative and conjugative element in Streptococcus mutans that when expressed causes growth arrest of host bacteria.Molecular microbiology · 2022Article
- Activation of the integrative and conjugative element Tn916 causes growth arrest and death of host bacteria.PLoS genetics · 2022Article
- A CRISPR interference screen reveals a role for cell wall teichoic acids in conjugation in Bacillus subtilis.Molecular microbiology · 2022Article
- Specificity and Selective Advantage of an Exclusion System in the Integrative and Conjugative Element ICEJournal of bacteriology · 2021Article
- Article
- Identification, characterization and benefits of an exclusion system in an integrative and conjugative element of Bacillus subtilis.Molecular microbiology · 2019Article
- The hidden life of integrative and conjugative elements.FEMS microbiology reviews · 2017Review
- Highly variable individual donor cell fates characterize robust horizontal gene transfer of an integrative and conjugative element.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Identification of a Single Strand Origin of Replication in the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis.PLoS genetics · 2015Article
- Intermediate Levels of Bacillus subtilis CodY Activity Are Required for Derepression of the Branched-Chain Amino Acid Permease, BraB.PLoS genetics · 2015Article
- Critical Components of the Conjugation Machinery of the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis.Journal of bacteriology · 2015Article
- Delineation of polar localization domains of Agrobacterium tumefaciens type IV secretion apparatus proteins VirB4 and VirB11.MicrobiologyOpen · 2014Article
- Identification of host genes that affect acquisition of an integrative and conjugative element in Bacillus subtilis.Molecular microbiology · 2014Article
- The bifunctional cell wall hydrolase CwlT is needed for conjugation of the integrative and conjugative element ICEBs1 in Bacillus subtilis and B. anthracis.Journal of bacteriology · 2014Article
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4 authors at 2 institutions in 1 country.
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Abstract
ICEBs1 is an integrative and conjugative element found in the chromosome of Bacillus subtilis. ICEBs1 encodes functions needed for its excision and transfer to recipient cells. We found that the ICEBs1 gene conE (formerly yddE) is required for conjugation and that conjugative transfer of ICEBs1 requires a conserved ATPase motif of ConE. ConE belongs to the HerA/FtsK superfamily of ATPases, which includes the well-characterized proteins FtsK, SpoIIIE, VirB4, and VirD4. We found that a ConE-GFP (green fluorescent protein) fusion associated with the membrane predominantly at the cell poles in ICEBs1 donor cells. At least one ICEBs1 product likely interacts with ConE to target it to the membrane and cell poles, as ConE-GFP was dispersed throughout the cytoplasm in a strain lacking ICEBs1. We also visualized the subcellular location of ICEBs1. When integrated in the chromosome, ICEBs1 was located near midcell along the length of the cell, a position characteristic of that chromosomal region. Following excision, ICEBs1 was more frequently found near a cell pole. Excision of ICEBs1 also caused altered positioning of at least one component of the replisome. Taken together, our findings indicate that ConE is a critical component of the ICEBs1 conjugation machinery, that conjugative transfer of ICEBs1 from B. subtilis likely initiates at a donor cell pole, and that ICEBs1 affects the subcellular position of the replisome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.