ArticleArchives of biochemistry and biophysics2009
Inactivation of cystathionine beta-synthase with peroxynitrite.
Article in Archives of biochemistry and biophysics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- A Paradigm Shift of HCurrent issues in molecular biology · 2026Review
- Posttranslational Regulation of Mammalian Sulfur Amino Acid Metabolism.International journal of molecular sciences · 2025Review
- Article
- Blockade of the trans-sulfuration pathway in acute pancreatitis due to nitration of cystathionine β-synthase.Redox biology · 2020Article
- Allosteric control of human cystathionine β-synthase activity by a redox active disulfide bond.The Journal of biological chemistry · 2018Article
- Hydrogen Sulfide Biochemistry and Interplay with Other Gaseous Mediators in Mammalian Physiology.Oxidative medicine and cellular longevity · 2018Review
- Kinetics of Nitrite Reduction and Peroxynitrite Formation by Ferrous Heme in Human Cystathionine β-Synthase.The Journal of biological chemistry · 2016Article
- S-glutathionylation enhances human cystathionine β-synthase activity under oxidative stress conditions.Antioxidants & redox signaling · 2015Article
- Alleviation of plasma homocysteine level by phytoestrogen α-zearalanol might be related to the reduction of cystathionine β-synthase nitration.BioMed research international · 2014Article
- Kinetics of reversible reductive carbonylation of heme in human cystathionine β-synthase.Biochemistry · 2013Article
- Comparative study of enzyme activity and heme reactivity in Drosophila melanogaster and Homo sapiens cystathionine β-synthases.Biochemistry · 2013Article
- Article
- Cobalt cystathionine β-synthase: a cobalt-substituted heme protein with a unique thiolate ligation motif.Inorganic chemistry · 2011Article
- Redox biochemistry of hydrogen sulfide.The Journal of biological chemistry · 2010Article
- Interactions of multiple gas-transducing systems: hallmarks and uncertainties of CO, NO, and H2S gas biology.Antioxidants & redox signaling · 2010Review
- Neuroprotection by radical avoidance: search for suitable agents.Molecules (Basel, Switzerland) · 2009Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Cystathionine beta-synthase (CBS) is a homocysteine metabolizing enzyme that contains pyridoxal phosphate (PLP) and a six-coordinate heme cofactor of unknown function. CBS was inactivated by peroxynitrite, the product of nitric oxide and superoxide radicals. The IC(50) was approximately 150microM for 5microM ferric CBS. Stopped-flow kinetics and competition experiments showed a direct reaction with a second-order rate constant of (2.4-5.0)x10(4)M(-1)s(-1) (pH 7.4, 37 degrees C). The radicals derived from peroxynitrite, nitrogen dioxide and carbonate radical, also inactivated CBS. Exposure to peroxynitrite did not modify bound PLP but led to nitration of Trp208, Trp43 and Tyr223 and alterations in the heme environment including loss of thiolate coordination, conversion to high-spin and bleaching, with no detectable formation of oxo-ferryl compounds nor promotion of one-electron processes. This study demonstrates the susceptibility of CBS to reactive oxygen/nitrogen species, with potential relevance to hyperhomocysteinemia, a risk factor for cardiovascular diseases.
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Registered trials
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