ArticleBMC genomics2009
Isolation and sequence analysis of the wheat B genome subtelomeric DNA.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Bread wheat satellitome: a complex scenario in a huge genome.Plant molecular biology · 2024Article
- Telomeres and Subtelomeres Dynamics in the Context of Early Chromosome Interactions During Meiosis and Their Implications in Plant Breeding.Frontiers in plant science · 2021Review
- Sequence analysis of wheat subtelomeres reveals a high polymorphism among homoeologous chromosomes.The plant genome · 2020Article
- Supernumerary B Chromosomes and Plant Genome Changes: A Snapshot of Wild Populations ofInternational journal of molecular sciences · 2020Article
- GISH-based comparative genomic analysis in Urochloa P. Beauv.Molecular biology reports · 2020Article
- Cytological markers used for identification and transfer ofComparative cytogenetics · 2019Review
- Intraspecific and intraorganismal copy number dynamics of retrotransposons and tandem repeat in Aegilops speltoides Tausch (Poaceae, Triticeae).Protoplasma · 2018Article
- Article
- Transposable Elements in the Organization and Diversification of the Genome ofInternational journal of genomics · 2018Article
- Evolution of the S-Genomes inFrontiers in plant science · 2018Article
- Similarities and differences in the nuclear genome organization within Pooideae species revealed by comparative genomic in situ hybridization (GISH).Journal of applied genetics · 2017Article
- Intraspecific Polymorphisms of Cytogenetic Markers Mapped on Chromosomes of Triticum polonicum L.PloS one · 2016Article
- Mapping the 'breaker' element of the gametocidal locus proximal to a block of sub-telomeric heterochromatin on the long arm of chromosome 4S(sh) of Aegilops sharonensis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Introgression of A- and B-genome of tetraploid triticale chromatin into tetraploid rye.Journal of applied genetics · 2013Article
- Cytogenetic analysis of Aegilops chromosomes, potentially usable in triticale (X Triticosecale Witt.) breeding.Journal of applied genetics · 2013Article
- The impact of Ty3-gypsy group LTR retrotransposons Fatima on B-genome specificity of polyploid wheats.BMC plant biology · 2011Article
- Evolutionary analysis of the CACTA DNA-transposon Caspar across wheat species using sequence comparison and in situ hybridization.Molecular genetics and genomics : MGG · 2010Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundTelomeric and subtelomeric regions are essential for genome stability and regular chromosome replication. In this work, we have characterized the wheat BAC (bacterial artificial chromosome) clones containing Spelt1 and Spelt52 sequences, which belong to the subtelomeric repeats of the B/G genomes of wheats and Aegilops species from the section Sitopsis.
resultsThe BAC library from Triticum aestivum cv. Renan was screened using Spelt1 and Spelt52 as probes. Nine positive clones were isolated; of them, clone 2050O8 was localized mainly to the distal parts of wheat chromosomes by in situ hybridization. The distribution of the other clones indicated the presence of different types of repetitive sequences in BACs. Use of different approaches allowed us to prove that seven of the nine isolated clones belonged to the subtelomeric chromosomal regions. Clone 2050O8 was sequenced and its sequence of 119,737 bp was annotated. It is composed of 33% transposable elements (TEs), 8.2% Spelt52 (namely, the subfamily Spelt52.2) and five non-TE-related genes. DNA transposons are predominant, making up 24.6% of the entire BAC clone, whereas retroelements account for 8.4% of the clone length. The full-length CACTA transposon Caspar covers 11,666 bp, encoding a transposase and CTG-2 proteins, and this transposon accounts for 40% of the DNA transposons. The in situ hybridization data for 2050O8 derived subclones in combination with the BLAST search against wheat mapped ESTs (expressed sequence tags) suggest that clone 2050O8 is located in the terminal bin 4BL-10 (0.95-1.0). Additionally, four of the predicted 2050O8 genes showed significant homology to four putative orthologous rice genes in the distal part of rice chromosome 3S and confirm the synteny to wheat 4BL.
conclusionSatellite DNA sequences from the subtelomeric regions of diploid wheat progenitor can be used for selecting the BAC clones from the corresponding regions of hexaploid wheat chromosomes. It has been demonstrated for the first time that Spelt52 sequences were involved in the evolution of terminal regions of common wheat chromosomes. Our research provides new insights into the microcollinearity in the terminal regions of wheat chromosomes 4BL and rice chromosome 3S.
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