ArticleCellular microbiology2010
A highly sensitive FRET-based approach reveals secretion of the actin-binding protein toxofilin during Toxoplasma gondii infection.
Article in Cellular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 66 citations in OpenAlex.
- Clustering of host N-glycans by the microneme MIC1/4/6 complex licenses Toxoplasma rhoptry discharge.The EMBO journal · 2026Article
- CRISPR-based functional profiling of the Toxoplasma gondii genome during acute murine infection.Nature microbiology · 2024Article
- Toxoplasma gondii infection supports the infiltration of T cells into brain tumors.Journal of neuroimmunology · 2024Article
- Harnessing a brain parasite as a tool for delivery of therapeutics to the brain.Nature microbiology · 2024Article
- Engineering Toxoplasma gondii secretion systems for intracellular delivery of multiple large therapeutic proteins to neurons.Nature microbiology · 2024Article
- Monitoring Effector Translocation with the TEM-1 Beta-Lactamase Reporter System: From Endpoint to Time Course Analysis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Ceramide biosynthesis is critical for establishment of the intracellular niche of Toxoplasma gondii.Cell reports · 2022Article
- The enteric pathogeneLife · 2021Article
- Toxoplasma gondii infection and its implications within the central nervous system.Nature reviews. Microbiology · 2021Review
- Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion.Nature communications · 2021Article
- Review
- Article
- Translocation of effector proteins into host cells by Toxoplasma gondii.Current opinion in microbiology · 2019Review
- Vaccination with toxofilin DNA in combination with an alum-monophosphoryl lipid A mixed adjuvant induces significant protective immunity against Toxoplasma gondii.BMC infectious diseases · 2017Article
- Characterization of Toxoplasma DegP, a rhoptry serine protease crucial for lethal infection in mice.PloS one · 2017Article
- Article
- Identification of three novel Toxoplasma gondii rhoptry proteins.International journal for parasitology · 2014Article
- Identification of differentially expressed proteins in sulfadiazine resistant and sensitive strains of Toxoplasma gondii using difference-gel electrophoresis (DIGE).International journal for parasitology. Drugs and drug resistance · 2013Article
- Small-molecule inhibition of a depalmitoylase enhances Toxoplasma host-cell invasion.Nature chemical biology · 2013Article
- Human innate immunity to Toxoplasma gondii is mediated by host caspase-1 and ASC and parasite GRA15.mBio · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have utilized a highly sensitive approach based on fluorescence resonance energy transfer (FRET) and beta-lactamase (BLA), which we adapted for the detection of Toxoplasma gondii secreted proteins. This assay revealed that the actin-binding protein toxofilin appears to be secreted into host cells during invasion. To determine the function of toxofilin during infection, we engineered a type I (RH strain) parasite with a targeted deletion of the toxofilin gene and compared the phenotypes of control and toxofilin knockout (Deltatxf) parasites in several in vitro assays, including invasion, growth, gliding motility, and egress of the Deltatxf parasites, as well as F-actin staining, phagocytosis and migration of cells infected with Deltatxf parasites or wild-type controls. Despite its apparent secretion into host cells and its ability to bind to and modulate host actin, we observed that toxofilin does not appear to play a role in these processes, under the conditions we examined, and we report these findings here.
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