ArticleCurrent protocols in cell biology2009
Generation of gene knockout mice by ES cell microinjection.
Article in Current protocols in cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Pathogenic variants in SMARCA1 cause an X-linked neurodevelopmental disorder modulated by NURF complex composition.Nature communications · 2025Article
- Animal Models of Uveal Melanoma.Annals of eye science · 2022Article
- Transgenic mice with an R342X mutation in Phf6 display clinical features of Börjeson-Forssman-Lehmann Syndrome.Human molecular genetics · 2021Article
- Cryopreservation Protocols for Genetically Engineered Mice.Current protocols · 2021Article
- Arsenic Metabolism in Mice Carrying aEnvironmental health perspectives · 2020Article
- An Improved Method for Differentiating Mouse Embryonic Stem Cells into Cerebellar Purkinje Neurons.Cerebellum (London, England) · 2019Article
- Thick Ascending Limb Sodium Transport in the Pathogenesis of Hypertension.Physiological reviews · 2019Review
- Genome Editing in Mice Using CRISPR/Cas9 Technology.Current protocols in cell biology · 2018Article
- Article
- Generation of Mitochondrial-nuclear eXchange Mice via Pronuclear Transfer.Bio-protocol · 2016Article
- Biallelic Mutations in PDE10A Lead to Loss of Striatal PDE10A and a Hyperkinetic Movement Disorder with Onset in Infancy.American journal of human genetics · 2016Article
- Derivation and maintenance of murine trophoblast stem cells under defined conditions.Stem cell reports · 2014Article
- SIRT1 regulates differentiation of mesenchymal stem cells by deacetylating β-catenin.EMBO molecular medicine · 2013Article
- Gene targeting in mice: a review.Methods in molecular biology (Clifton, N.J.) · 2013Review
- Nonsurgical embryo transfer device compared with surgery for embryo transfer in mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
This unit describes protocols used in the production of chimeric mice that are then used for the generation of gene knockout mice. These protocols include the collection of blastocyst embryos, ES cell injection, and uterine transfer of injected blastocysts. Support protocols for the superovulation of blastocyst donor mice, generation of pseudopregnant recipients, fabrication of glass pipets for embryo and cell manipulations, and generation of germline mice are also included. Practical tips and solutions are mentioned to help troubleshoot problems that may occur.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.