ArticleCell death and differentiation2010
Features of programmed cell death in intact Xenopus oocytes and early embryos revealed by near-infrared fluorescence and real-time monitoring.
Article in Cell death and differentiation, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- TheBiomolecules · 2025Review
- Prolonged hypothermic storage of oocytes of the European common frog Rana temporaria in a gas mixture of oxygen and carbon monoxide.PloS one · 2023Article
- Article
- An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos.The Journal of experimental biology · 2022Article
- Emerin induces nuclear breakage inMolecular biology of the cell · 2018Article
- BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins.American journal of physiology. Cell physiology · 2015Article
- Cell death stages in single apoptotic and necrotic cells monitored by Raman microspectroscopy.Scientific reports · 2014Article
- A voltage dependent non-inactivating Na+ channel activated during apoptosis in Xenopus oocytes.PloS one · 2014Article
- An increase of granulosa cell apoptosis mediates aqueous neem (Azadirachta indica) leaf extract-induced oocyte apoptosis in rat.International journal of applied & basic medical research · 2013Article
- Quality improvement in gastroenterology clinical practice.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2012Review
- Review
- Aqueous extract of Azadirachta indica (neem) leaf induces generation of reactive oxygen species and mitochondria-mediated apoptosis in rat oocytes.Journal of assisted reproduction and genetics · 2012Article
- Unfertilized frog eggs die by apoptosis following meiotic exit.BMC cell biology · 2011Article
- Intracellular K+ concentration decrease is not obligatory for apoptosis.The Journal of biological chemistry · 2011Article
- Anti-apoptotic activity and proteasome-mediated degradation of Xenopus Mcl-1 protein in egg extracts.The Journal of biological chemistry · 2011Article
- Mitochondrial apoptosis-induced channel (MAC) function triggers a Bax/Bak-dependent bystander effect.The American journal of pathology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Factors influencing apoptosis of vertebrate eggs and early embryos have been studied in cell-free systems and in intact embryos by analyzing individual apoptotic regulators or caspase activation in static samples. A novel method for monitoring caspase activity in living Xenopus oocytes and early embryos is described here. The approach, using microinjection of a near-infrared caspase substrate that emits fluorescence only after its proteolytic cleavage by active effector caspases, has enabled the elucidation of otherwise cryptic aspects of apoptotic regulation. In particular, we show that brief caspase activity (10 min) is sufficient to cause apoptotic death in this system. We illustrate a cytochrome c dose threshold in the oocyte, which is lowered by Smac, a protein that binds thereby neutralizing the inhibitor of apoptosis proteins. We show that meiotic oocytes develop resistance to cytochrome c, and that the eventual death of oocytes arrested in meiosis is caspase-independent. Finally, data acquired through imaging caspase activity in the Xenopus embryo suggest that apoptosis in very early development is not cell-autonomous. These studies both validate this assay as a useful tool for apoptosis research and reveal subtleties in the cell death program during early development. Moreover, this method offers a potentially valuable screening modality for identifying novel apoptotic regulators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.