ArticleMicrobiology (Reading, England)2009
N-acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila.
Article in Microbiology (Reading, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 143 citations in OpenAlex.
- Emodin treatment is associated with enhanced resistance toFrontiers in immunology · 2026Article
- The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin.Microbial biotechnology · 2025Article
- Impact of Quorum Sensing on the Virulence and Survival Traits ofPlants (Basel, Switzerland) · 2024Article
- A coordinated attack by a bacterial secretion system and a small molecule drives prey specificity.Communications biology · 2024Article
- Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing.Applied microbiology and biotechnology · 2024Review
- Transcriptome profiling of type VI secretion system core geneMicrobiology spectrum · 2024Article
- Carvacrol Inhibits Quorum Sensing in Opportunistic BacteriumMicroorganisms · 2023Article
- Regulation of type VI secretion systems at the transcriptional, posttranscriptional and posttranslational level.Microbiology (Reading, England) · 2023Review
- In Vitro Antibiofilm Activity of Resveratrol againstAntibiotics (Basel, Switzerland) · 2023Article
- Heterologous expression of quorum sensing transcriptional regulator LitR and its function in virulence-related gene regulation in foodborne pathogen Aeromonas hydrophila.Molecular biology reports · 2023Article
- A Quorum Sensing-Regulated Type VI Secretion System Containing Multiple Nonredundant VgrG Proteins Is Required for Interbacterial Competition in Chromobacterium violaceum.Microbiology spectrum · 2022Article
- Innovative microbial disease biocontrol strategies mediated by quorum quenching and their multifaceted applications: A review.Frontiers in plant science · 2022Review
- Structural insights into acyl-ACP selective recognition by the Aeromonas hydrophila AHL synthase AhyI.BMC microbiology · 2021Article
- Attenuation ofAntibiotics (Basel, Switzerland) · 2021Article
- Differential production and secretion of potentially toxigenic extracellular proteins from hypervirulent Aeromonas hydrophila under biofilm and planktonic culture.BMC microbiology · 2021Article
- Inhibition of Quorum Sensing and Biofilm Formation of Esculetin onFrontiers in microbiology · 2021Article
- Heterologous expression of AHL lactonase AiiK by Lactobacillus casei MCJΔ1 with great quorum quenching ability against Aeromonas hydrophila AH-1 and AH-4.Microbial cell factories · 2020Article
- Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control.The Journal of biological chemistry · 2020Review
- The Marine Bacterium Shewanella woodyi Produces CMicrobial ecology · 2020Article
- Effect of curcumin on the expression of ahyI/R quorum sensing genes and some associated phenotypes in pathogenic Aeromonas hydrophila fish isolates.World journal of microbiology & biotechnology · 2020Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
In this study, we delineated the role of N-acylhomoserine lactone(s) (AHLs)-mediated quorum sensing (QS) in the virulence of diarrhoeal isolate SSU of Aeromonas hydrophila by generating a double knockout Delta ahyRI mutant. Protease production was substantially reduced in the Delta ahyRI mutant when compared with that in the wild-type (WT) strain. Importantly, based on Western blot analysis, the Delta ahyRI mutant was unable to secrete type VI secretion system (T6SS)-associated effectors, namely haemolysin coregulated protein and the valine-glycine repeat family of proteins, while significant levels of these effectors were detected in the culture supernatant of the WT A. hydrophila. In contrast, the production and translocation of the type III secretion system (T3SS) effector AexU in human colonic epithelial cells were not affected when the ahyRI genes were deleted. Solid surface-associated biofilm formation was significantly reduced in the Delta ahyRI mutant when compared with that in the WT strain, as determined by a crystal violet staining assay. Scanning electron microscopic observations revealed that the Delta ahyRI mutant was also defective in the formation of structured biofilm, as it was less filamentous and produced a distinct exopolysaccharide on its surface when compared with the structured biofilm produced by the WT strain. These effects of AhyRI could be complemented either by expressing the ahyRI genes in trans or by the exogeneous addition of AHLs to the Delta ahyRI/ahyR(+) complemented strain. In a mouse lethality experiment, 50 % attenuation was observed when we deleted the ahyRI genes from the parental strain of A. hydrophila. Together, our data suggest that AHL-mediated QS modulates the virulence of A. hydrophila SSU by regulating the T6SS, metalloprotease production and biofilm formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.