ArticleProtein engineering, design & selection : PEDS2009
Towards evolving a better repressor.
Article in Protein engineering, design & selection : PEDS, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Rugged fitness landscapes minimize promiscuity in the evolution of transcriptional repressors.Cell systems · 2024Article
- SirA inhibits the essential DnaA:DnaD interaction to block helicase recruitment during Bacillus subtilis sporulation.Nucleic acids research · 2023Article
- Practical genetic control strategies for industrial bioprocesses.Journal of industrial microbiology & biotechnology · 2022Review
- Engineered signal-coupled inducible promoters: measuring the apparent RNA-polymerase resource budget.Nucleic acids research · 2020Article
- Predictive shifts in free energy couple mutations to their phenotypic consequences.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Long-range transcriptional interference in E. coli used to construct a dual positive selection system for genetic switches.Nucleic acids research · 2016Article
- A quantitative understanding of lac repressor's binding specificity and flexibility.Quantitative biology (Beijing, China) · 2015Article
- Napoleon Is in Equilibrium.Annual review of condensed matter physics · 2015Article
- High-resolution specificity from DNA sequencing highlights alternative modes of Lac repressor binding.Genetics · 2014Article
- In vitro transcription accurately predicts lac repressor phenotype in vivo in Escherichia coli.PeerJ · 2014Article
- Identification of an intestine-specific promoter and inducible expression of bacterial α-galactosidase in mammalian cells by a lac operon system.Journal of animal science and biotechnology · 2012Article
- Thermodynamic analysis of mutant lac repressors.Journal of molecular biology · 2011Article
- Local gene regulation details a recognition code within the LacI transcriptional factor family.PLoS computational biology · 2010Article
- Rational design of modular circuits for gene transcription: A test of the bottom-up approach.Journal of biological engineering · 2010Article
- Salmonella vaccine vectors displaying delayed antigen synthesis in vivo to enhance immunogenicity.Infection and immunity · 2010Article
- Functional rules for lac repressor-operator associations and implications for protein-DNA interactions.Protein science : a publication of the Protein Society · 2010Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Transcriptional regulation is an essential component of all metabolic pathways. At the most basic level, a protein binds to a particular DNA sequence (operator) on the genome and either positively or negatively alters the level of transcription. Together, the protein and its operator form an epigenetic switch that regulates gene expression. In an effort to produce a 'better' switch, we have discovered novel facets of the lac operon that are responsible for optimal functionality. We have uncovered a relationship between operator binding affinity and inducibility and demonstrated that the operator DNA is not a passive component of a genetic switch; it is responsible for establishing binding affinity, specificity as well as translational efficiency. In addition, an operator's directionality can indirectly affect gene expression. Unraveling the basic properties of this classical epigenetic switch demonstrates that multiple factors must be optimized in designing a better switch.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.