ArticleJournal of virological methods2009
A real-time TaqMan RT-PCR assay with an internal amplification control for rapid detection of transmissible gastroenteritis virus in swine fecal samples.
Article in Journal of virological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Prevalence and Genetic Characterization of Porcine Respiratory Coronavirus in Korean Pig Farms.Animals : an open access journal from MDPI · 2024Article
- SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters.Water research X · 2024Article
- A high-performance polymer composite column for coronavirus nucleic acid purification.Scientific reports · 2024Article
- Analysis of SARS-CoV-2 in wastewater for prevalence estimation and investigating clinical diagnostic test biases.Water research · 2023Article
- Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses.Pathogens (Basel, Switzerland) · 2023Article
- Development and Clinical Applications of a 5-Plex Real-Time RT-PCR for Swine Enteric Coronaviruses.Viruses · 2022Article
- Dynamics of SARS-CoV-2 Alpha (B.1.1.7) variant spread: The wastewater surveillance approach.Environmental research · 2022Article
- Monitoring Emergence of the SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19).Environmental science & technology · 2021Article
- Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic.International journal of environmental research and public health · 2020Article
- Rapid and efficient detection methods of pathogenic swine enteric coronaviruses.Applied microbiology and biotechnology · 2020Review
- Glass Wool Concentration Optimization for the Detection of Enveloped and Non-enveloped Waterborne Viruses.Food and environmental virology · 2019Article
- Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification.Journal of virological methods · 2018Article
- No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs.BMC veterinary research · 2017Article
- The first case of porcine epidemic diarrhea in Canada.The Canadian veterinary journal = La revue veterinaire canadienne · 2015Article
- Ultrasensitive Detection of RNA and DNA Viruses Simultaneously Using Duplex UNDP-PCR Assay.PloS one · 2015Article
- Attempted experimental reproduction of porcine periweaning-failure-to-thrive syndrome using tissue homogenates.PloS one · 2014Article
- Generation of a mouse scFv library specific for porcine aminopeptidase N using the T7 phage display system.Journal of virological methods · 2012Article
- Reverse transcription loop-mediated isothermal amplification for rapid detection of transmissible gastroenteritis virus.Current microbiology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A TaqMan probe-based real-time RT-PCR assay was developed for simultaneous detection of RNA of transmissible gastroenteritis virus (TGEV) in pig fecal samples and RNA of enhanced green fluorescent protein (EGFP) added exogenously as an internal amplification control. The TGEV primers and probe were designed to be specific to a portion of the S gene sequence conserved in all TGEV isolates, but absent in the closely related porcine respiratory coronaviruses. The optimized TaqMan assay detected a minimum of 2.8 copies of in vitro transcribed RNA of the target S gene and RNA extracted from 1 TCID50/ml of TGEV. Using 113 clinical samples received at our diagnostic laboratory over a 4-year period, the performance of the assay was tested and compared with that of a previously described nested RT-PCR assay. All the fecal samples which tested positive for TGEV by the nested RT-PCR assay also tested positive by the TaqMan assay. However, approximately 9% of the samples that tested negative by the nested RT-PCR assay tested positive by the TaqMan assay. These results indicate that the developed TaqMan assay is a highly sensitive diagnostic test for rapid detection of TGEV in pig fecal samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.