ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2009
Development of a real-time PCR for the specific detection of Waddlia chondrophila in clinical samples.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 85 citations in OpenAlex.
- Review
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- Pharmacophore modelling based virtual screening and molecular dynamics identified the novel inhibitors and drug targets against Waddlia chondrophila.Scientific reports · 2024Article
- Temperature Affects the Host Range of Rhabdochlamydia porcellionis.Applied and environmental microbiology · 2023Article
- Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion.Pathogens (Basel, Switzerland) · 2023Article
- Subtractive proteomics approach to Unravel the druggable proteins of the emerging pathogenHeliyon · 2021Article
- COVID-19: Diagnostics, Therapeutic Advances, and Vaccine Development.Current clinical microbiology reports · 2021Review
- Evaluations of the serological test in the diagnosis of 2019 novel coronavirus (SARS-CoV-2) infections during the COVID-19 outbreak.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
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- Eukaryotic Cell Permeabilisation to Identify New Putative Chlamydial Type III Secretion System Effectors Secreted within Host Cell Cytoplasm.Microorganisms · 2020Article
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- Impact ofNew microbes and new infections · 2020Article
- A predation assay using amoebae to screen for virulence factors unearthed the first W. chondrophila inclusion membrane protein.Scientific reports · 2019Article
- Interactions Screenings Unearth Potential New Divisome Components in theMicroorganisms · 2019Article
- Article
- Article
- Identification of new DNA-associated proteins from Waddlia chondrophila.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Waddlia chondrophila is considered as an emerging human pathogen likely involved in miscarriage and lower respiratory tract infections. Given the low sensitivity of cell culture to recover such an obligate intracellular bacteria, molecular-based diagnostic approaches are warranted. We thus developed a real-time PCR that amplifies Waddlia chondrophila DNA. Specific primers and probe were selected to target the 16S rRNA gene. The PCR specifically amplified W. chondrophila but did not amplify other related-bacteria such as Parachlamydia acanthamoebae, Simkania negevensis and Chlamydia pneumoniae. The PCR exhibited a good intra-run and inter-run reproducibility and a sensitivity of less than ten copies of the positive control. This real-time PCR was then applied to 32 nasopharyngeal aspirates taken from children with bronchiolitis not due to respiratory syncytial virus (RSV). Three samples revealed to be Waddlia positive, suggesting a possible role of this Chlamydia-related bacteria in this setting.
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Registered trials
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