ArticleGene therapy2010
Autoregulatory lentiviral vectors allow multiple cycles of doxycycline-inducible gene expression in human hematopoietic cells in vivo.
Article in Gene therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Engineering B cells with customized therapeutic responses using a synthetic circuit.Molecular therapy. Nucleic acids · 2023Article
- Review
- Sixth European Seminar in Virology on Virus⁻Host Interaction at Single Cell and Organism Level.Viruses · 2018Article
- Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion.Clinical oral investigations · 2018Article
- Helicobacter pylori gene silencing in vivo demonstrates urease is essential for chronic infection.PLoS pathogens · 2017Article
- SOCS1 function in BCR-ABL mediated myeloproliferative disease is dependent on the cytokine environment.PloS one · 2017Article
- Non-coding RNA LINC00857 is predictive of poor patient survival and promotes tumor progression via cell cycle regulation in lung cancer.Oncotarget · 2016Article
- Tet-On Systems For Doxycycline-inducible Gene Expression.Current gene therapy · 2016Review
- Tightly regulated 'all-in-one' lentiviral vectors for protection of human hematopoietic cells from anticancer chemotherapy.Gene therapy · 2015Article
- Safety mechanism assisted by the repressor of tetracycline (SMART) vaccinia virus vectors for vaccines and therapeutics.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Preclinical in vivo evaluation of the safety of a multi-shRNA-based gene therapy against HIV-1.Molecular therapy. Nucleic acids · 2013Article
- Graded or threshold response of the tet-controlled gene expression: all depends on the concentration of the transactivator.BMC biotechnology · 2013Article
- A doxycycline-dependent human immunodeficiency virus type 1 replicates in vivo without inducing CD4+ T-cell depletion.The Journal of general virology · 2012Article
- Selection of RNAi-based inhibitors for anti-HIV gene therapy.World journal of virology · 2012Review
- Adeno-associated virus serotype 2 mediated transduction and coexpression of the human apoAI and SR-BI gene in HepG2 cells.Molecular biology reports · 2012Article
- Cytosolic CD38 protein forms intact disulfides and is active in elevating intracellular cyclic ADP-ribose.The Journal of biological chemistry · 2011Article
- Separating lentiviral vector injection and induction of gene expression in time, does not prevent an immune response to rtTA in rats.PloS one · 2010Article
- Article
- Humanized mouse models of HIV infection.AIDS reviewsReview
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
The efficient control of gene expression in vivo from lentiviral vectors remains technically challenging. To analyze inducible gene expression in a human setting, we generated 'human immune system' (HIS) mice by transplanting newborn BALB/c Rag2(-/-)IL-2Rgamma(c)(-/-) immunodeficient mice with human hematopoietic stem cells transduced with a doxycycline-inducible lentiviral vector. We compared several methods of doxycycline delivery to mice, and could accurately measure doxycycline in vivo using a new sensitive detection assay. Two different lentiviral vector designs with constitutive (TRECMV-V14) or autoregulatory (TREAuto-V14) expression of an optimized reverse tetracycline transactivator were used to transduce human hematopoietic stem cells. After transplantation into immunodeficient mice, we analyzed the expression of the green fluorescent protein (GFP) reporter gene in the human hematopoiesis-derived cells that develop and accumulate in the generated HIS mice. We show efficient inducible GFP expression in adult HIS mice containing TREAuto-V14-transduced human cells, whereas GFP expression is poor with the TRECMV-V14 vector. Multiple cycles of doxycycline exposure in the TREAuto-V14 group result in repeated cycles of GFP expression with no loss of intensity. These findings are of major interest for gene therapy and basic research settings that require inducible gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.