ArticleJournal of biomolecular screening2009
Live-cell imaging of caspase activation for high-content screening.
Article in Journal of biomolecular screening, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Gain of 20q11.21 in human pluripotent stem cells enhances differentiation to retinal pigment epithelium.Stem cell research & therapy · 2025Article
- A Comprehensive Exploration of Caspase Detection Methods: From Classical Approaches to Cutting-Edge Innovations.International journal of molecular sciences · 2024Review
- Chromatin compaction precedes apoptosis in developing neurons.Communications biology · 2022Article
- A single-cell analytical approach to quantify activated caspase-3/7 during osteoblast proliferation, differentiation, and apoptosis.Analytical and bioanalytical chemistry · 2021Article
- 4-Hydroxy-7-oxo-5-heptenoic acid (HOHA) lactone induces apoptosis in retinal pigment epithelial cells.Free radical biology & medicine · 2020Article
- A mechanism for increased sensitivity of acute myeloid leukemia to mitotoxic drugs.Cell death & disease · 2019Article
- A nonalcoholic fatty liver disease model in human induced pluripotent stem cell-derived hepatocytes, created by endoplasmic reticulum stress-induced steatosis.Disease models & mechanisms · 2018Article
- Review: Microbial analysis in dielectrophoretic microfluidic systems.Analytica chimica acta · 2017Review
- A High-Content Screening Technology for Quantitatively Studying Podocyte Dynamics.Advances in chronic kidney disease · 2017Review
- Increasing the Content of High-Content Screening: An Overview.Journal of biomolecular screening · 2014Review
- A high content assay to assess cellular fitness.Combinatorial chemistry & high throughput screening · 2014Article
- Remedial applications of silencing ribonucleic acids and modalities for its delivery to the kidneys--a review.African journal of traditional, complementary, and alternative medicines : AJTCAM · 2014Review
- An arrayed genome-scale lentiviral-enabled short hairpin RNA screen identifies lethal and rescuer gene candidates.Assay and drug development technologies · 2013Article
- Longitudinal bioluminescence imaging of the dynamics of Doxorubicin induced apoptosis.Theranostics · 2013Article
- Designs and concept reliance of a fully automated high-content screening platform.Journal of laboratory automation · 2012Article
- Monitoring cleaved caspase-3 activity and apoptosis of immortalized oligodendroglial cells using live-cell imaging and cleaveable fluorogenic-dye substrates following potassium-induced membrane depolarization.Journal of visualized experiments : JoVE · 2012Article
- Validation of a high-content screening assay using whole-well imaging of transformed phenotypes.Assay and drug development technologies · 2011Article
- High-content assay to identify inhibitors of dengue virus infection.Assay and drug development technologies · 2010Article
- Cytometry in cell necrobiology revisited. Recent advances and new vistas.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Review
- Cell death goes LIVE: technological advances in real-time tracking of cell death.Cell cycle (Georgetown, Tex.) · 2010Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Caspases are central to the execution of programmed cell death, and their activation constitutes the biochemical hallmark of apoptosis. In this article, the authors report the successful adaptation of a high-content assay method using the DEVDNucView488 fluorogenic substrate, and for the first time, they show caspase activation in live cells induced by either drugs or siRNA. The fluorogenic substrate was found to be nontoxic over an exposure period of several days, during which the authors demonstrate automated imaging and quantification of caspase activation of the same cell population as a function of time. Overexpression of the antiapoptotic protein Bcl-XL, alone or in combination with the inhibitor Z-VAD-FMK, attenuated caspase activation in HeLa cells exposed to doxorubicin, etoposide, or cell death siRNA. This method was further validated against 2 well-characterized NSCLC cell lines reported to be sensitive (H3255) or refractory (H2030) to erlotinib, where the authors show a differential time-dependent activation was observed for H3255 and no significant changes in H2030, consistent with their respective chemosensitivity profile. In summary, the results demonstrate the feasibility of using this newly adapted and validated high-content assay to screen chemical or RNAi libraries for the identification of previously uncovered enhancers and suppressors of the apoptotic machinery in live cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.