ArticleThe Journal of biological chemistry2009
Single-molecule analysis of proteinxDNA complexes formed during partition of newly replicated plasmid molecules in Streptococcus pyogenes.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Distinct architectural requirements for theeLife · 2022Article
- Article
- Single molecule analysis reveals monomeric XPA bends DNA and undergoes episodic linear diffusion during damage search.Nature communications · 2020Article
- Plasmid Localization and Partition inEcoSal Plus · 2019Review
- The TubR-centromere complex adopts a double-ring segrosome structure in Type III partition systems.Nucleic acids research · 2018Article
- Studying protein-DNA interactions using atomic force microscopy.Seminars in cell & developmental biology · 2018Review
- RecA Regulation by RecU and DprA DuringFrontiers in microbiology · 2018Article
- Segrosome Complex Formation during DNA Trafficking in Bacterial Cell Division.Frontiers in molecular biosciences · 2016Review
- The interaction of ω2 with the RNA polymerase β' subunit functions as an activation to repression switch.Nucleic acids research · 2015Article
- Molecular Anatomy of ParA-ParA and ParA-ParB Interactions during Plasmid Partitioning.The Journal of biological chemistry · 2015Article
- ParAB Partition Dynamics in Firmicutes: Nucleoid Bound ParA Captures and Tethers ParB-Plasmid Complexes.PloS one · 2015Article
- Interaction of branch migration translocases with the Holliday junction-resolving enzyme and their implications in Holliday junction resolution.The Journal of biological chemistry · 2014Article
- Bacillus subtilis DprA recruits RecA onto single-stranded DNA and mediates annealing of complementary strands coated by SsbB and SsbA.The Journal of biological chemistry · 2013Article
- VimA-dependent modulation of the secretome in Porphyromonas gingivalis.Molecular oral microbiology · 2012Article
- Functional characterization of the role of the chromosome I partitioning system in genome segregation in Deinococcus radiodurans.Journal of bacteriology · 2012Article
- Different phenotypes of Walker-like A box mutants of ParA homolog IncC of broad-host-range IncP plasmids.Plasmid · 2012Article
- Segrosome assembly at the pliable parH centromere.Nucleic acids research · 2011Article
- Molecular anatomy of the Streptococcus pyogenes pSM19035 partition and segrosome complexes.Nucleic acids research · 2011Article
- RecO-mediated DNA homology search and annealing is facilitated by SsbA.Nucleic acids research · 2010Article
- Role of vimA in cell surface biogenesis in Porphyromonas gingivalis.Microbiology (Reading, England) · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Streptococcus pyogenes pSM19035 partition locus is ubiquitous among plasmids from vancomycin- or methicillin-resistant bacteria. An increasing understanding of this segregation system may highlight novel protein targets that could be blocked to curb bacterial proliferation. pSM19035 segregation depends on two homodimeric (delta(2) (ParA) and omega(2) (ParB)) proteins and six cis-acting centromeric noncurved parS sites. In the presence of ATPxMg(2+), delta(2) (delta x ATP x Mg(2+))(2) binds DNA in a sequence-independent manner. Protein omega(2) binds with high affinity and cooperatively to B-form parS DNA. Atomic force microscopy experiments indicate that about 10 omega(2) molecules bind parS, consisting of 10 contiguous iterons. Protein (delta x ATP x Mg(2+))(2), by interacting with the N terminus of omega(2) bound to parS, loses its association with DNA and relocalizes with omega(2).parS to form a ternary complex ((deltaxATPxMg(2+))(2) x omega(2) x parS) with the DNA remaining in straight B-form. Then, the interaction of two (delta x ATP x Mg(2+))(2).omega(2).parS complexes via delta(2) promotes pairing of a plasmid subfraction. (deltaD60A x ATP x Mg(2+))(2), which binds but does not hydrolyze ATP, leads to accumulation of pairing intermediates, suggesting that ATP hydrolysis induces plasmid separation. We propose that the molar omega(2):delta(2) ratio regulates the different stages of pSM19035 segregation, pairing, and delta(2) polymerization, before cell division.
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