ArticleThe Journal of biological chemistry2009
Novel beta-propeller of the BTB-Kelch protein Krp1 provides a binding site for Lasp-1 that is necessary for pseudopodial extension.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- LASP1 in Cellular Signaling and Gene Expression: More than Just a Cytoskeletal Regulator.Cells · 2022Review
- Structural Model for Recruitment of RIT1 to the LZTR1 E3 Ligase: Evidences from an Integrated Computational Approach.Journal of chemical information and modeling · 2021Article
- Article
- NS1-binding protein radiosensitizes esophageal squamous cell carcinoma by transcriptionally suppressing c-Myc.Cancer communications (London, England) · 2018Article
- New Frontiers for the Cytoskeletal Protein LASP1.Frontiers in oncology · 2018Review
- Bioinformatics analysis of the proteins interacting with LASP-1 and their association with HBV-related hepatocellular carcinoma.Scientific reports · 2017Article
- Regulation of matrix metalloproteinases (MMPs) expression and secretion in MDA-MB-231 breast cancer cells by LIM and SH3 protein 1 (LASP1).Oncotarget · 2016Article
- Structural basis of Keap1 interactions with Nrf2.Free radical biology & medicine · 2015Review
- CDD: NCBI's conserved domain database.Nucleic acids research · 2015Article
- An update on the LIM and SH3 domain protein 1 (LASP1): a versatile structural, signaling, and biomarker protein.Oncotarget · 2015Review
- LASP1, a Newly Identified Melanocytic Protein with a Possible Role in Melanin Release, but Not in Melanoma Progression.PloS one · 2015Article
- Structural and biochemical characterization of the KLHL3-WNK kinase interaction important in blood pressure regulation.The Biochemical journal · 2014Article
- Identification of KLHL41 Mutations Implicates BTB-Kelch-Mediated Ubiquitination as an Alternate Pathway to Myofibrillar Disruption in Nemaline Myopathy.American journal of human genetics · 2013Article
- Structural basis for Cul3 protein assembly with the BTB-Kelch family of E3 ubiquitin ligases.The Journal of biological chemistry · 2013Article
- BTB-Kelch protein Krp1 regulates proliferation and differentiation of myoblasts.American journal of physiology. Cell physiology · 2011Article
- Scaffolds and chaperones in myofibril assembly: putting the striations in striated muscle.Biophysical reviews · 2011Article
- Review
- The Nance-Horan syndrome protein encodes a functional WAVE homology domain (WHD) and is important for co-ordinating actin remodelling and maintaining cell morphology.Human molecular genetics · 2010Article
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Authors and funding
5 authors.
Funding
Abstract
Kelch-related protein 1 (Krp1) is up-regulated in oncogene-transformed fibroblasts. The Kelch repeats interact directly with the actin-binding protein Lasp-1 in membrane ruffles at the tips of pseudopodia, where both proteins are necessary for pseudopodial elongation. Herein, we investigate the molecular basis for this interaction. Probing an array of overlapping decapeptides of Rattus norvegicus (Rat) Krp1 with recombinant Lasp-1 revealed two binding sites; one ((317)YDPMENECYLT(327)) precedes the first of five Kelch repeats, and the other ((563)TEVNDIWKYEDD(574)) is in the last of the five Kelch repeats. Mutational analysis established that both binding sites are necessary for Krp1-Lasp-1 interaction in vitro and function in vivo. The crystal structure of the C-terminal domain of rat Krp1 (amino acids 289-606) reveals that both binding sites are brought into close proximity by the formation of a novel six-bladed beta-propeller, where the first blade is not formed by a Kelch repeat.
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