ArticleThe Journal of biological chemistry2009
The transcriptional repressor CcpN from Bacillus subtilis uses different repression mechanisms at different promoters.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Review
- Small proteins in Gram-positive bacteria.FEMS microbiology reviews · 2023Article
- Microbial synthesis of bacitracin: Recent progress, challenges, and prospects.Synthetic and systems biotechnology · 2023Review
- A new role for SR1 from Bacillus subtilis: regulation of sporulation by inhibition of kinA translation.Nucleic acids research · 2021Article
- Review
- Intermolecular Communication inFrontiers in molecular biosciences · 2020Review
- EF1025, a Hypothetical Protein FromFrontiers in microbiology · 2020Article
- A new role for CsrA: promotion of complex formation between an sRNA and its mRNA target inRNA biology · 2019Article
- Dual-Function RNAs.Microbiology spectrum · 2018Review
- Stability of Proteins Out of Service: the GapB Case of Bacillus subtilis.Journal of bacteriology · 2017Article
- Dual-function sRNA encoded peptide SR1P modulates moonlighting activity of B. subtilis GapA.RNA biology · 2016Article
- Small regulatory RNAs from low-GC Gram-positive bacteria.RNA biology · 2014Review
- SR1--a small RNA with two remarkably conserved functions.Nucleic acids research · 2012Article
- Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CcpN, a transcriptional repressor from Bacillus subtilis that is responsible for the carbon catabolite repression of three genes, has been characterized in detail in the past 4 years. However, nothing is known about the actual repression mechanism as yet. Here, we present a detailed study on how CcpN exerts its repression effect at its three known target promoters of the genes sr1, pckA, and gapB. Using gel shift assays under non-repressive and repressive conditions, we showed that CcpN and RNA polymerase can bind simultaneously and that CcpN does not prevent RNA polymerase (RNAP) binding to the promoter. Furthermore, we investigated the effect of CcpN on open complex formation and demonstrate that CcpN also does not act at this step of transcription initiation at the sr1 and pckA and presumably at the gapB promoter. Investigation of abortive transcript synthesis revealed that CcpN acts differently at the three promoters: At the sr1 and pckA promoter, promoter clearance is impeded by CcpN, whereas synthesis of abortive transcripts is repressed at the gapB promoter. Eventually, we demonstrated with Far Western blots and co-elution experiments that CcpN is able to interact with the RNAP alpha-subunit, which completes the picture of the requirements for the repressive action of CcpN. On the basis of the presented results, we propose a new working model for CcpN action.
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