ArticleJournal of clinical microbiology2009
Design and validation of real-time reverse transcription-PCR assays for detection of pandemic (H1N1) 2009 virus.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Validation and comparison of EvaGreen- and TaqMan-based real-time qRT-PCR for diagnosis of the black queen cell virus in the honey bees.Veterinary research communications · 2025Article
- End-point RT-PCR based on a conservation landscape for SARS-COV-2 detection.Scientific reports · 2022Article
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- COVID-19 diagnosis -A review of current methods.Biosensors & bioelectronics · 2021Review
- Characterization of Swine Influenza A(H1N2) Variant, Alberta, Canada, 2020.Emerging infectious diseases · 2021Article
- A Recent Update on Advanced Molecular Diagnostic Techniques for COVID-19 Pandemic: An Overview.Frontiers in immunology · 2021Review
- Influenza virus detection in the stool of children with acute gastroenteritis.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
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- Could urine be useful for the diagnosis of Chlamydia trachomatis pneumonia in infancy?Diagnostic microbiology and infectious disease · 2014Article
- Detection of the pandemic H1N1/2009 influenza A virus by a highly sensitive quantitative real-time reverse-transcription polymerase chain reaction assay.Virologica Sinica · 2013Article
- Microdroplet sandwich real-time rt-PCR for detection of pandemic and seasonal influenza subtypes.PloS one · 2013Article
- Comparison of the Roche RealTime ready Influenza A/H1N1 Detection Set with CDC A/H1N1pdm09 RT-PCR on samples from three hospitals in Ho Chi Minh City, Vietnam.Diagnostic microbiology and infectious disease · 2012Article
- Update on influenza diagnostics: lessons from the novel H1N1 influenza A pandemic.Clinical microbiology reviews · 2012Review
- Two years after pandemic influenza A/2009/H1N1: what have we learned?Clinical microbiology reviews · 2012Review
- Article
- High success and low mortality rates with non-invasive ventilation in influenza A H1N1 patients in a tertiary hospital.BMC research notes · 2011Article
- Determination of the relative economic impact of different molecular-based laboratory algorithms for respiratory viral pathogen detection, including Pandemic (H1N1), using a secure web based platform.Virology journal · 2011Article
- Comparison of the Luminex xTAG respiratory viral panel with xTAG respiratory viral panel fast for diagnosis of respiratory virus infections.Journal of clinical microbiology · 2011Article
- Development of a real-time RT-PCR assay for detection of resistance to oseltamivir in influenza A pandemic (H1N1) 2009 virus using single nucleotide polymorphism probes.Journal of virological methods · 2011Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tracking novel influenza viruses which have the potential to cause pandemics, such as the pandemic (H1N1) 2009 virus, is a public health priority. Pandemic (H1N1) 2009 virus was first identified in Mexico in April 2009 and spread worldwide over a short period of time. Well-validated diagnostic tools that are rapid, sensitive, and specific for the detection and tracking of this virus are needed. Three real-time reverse transcription PCR (RT-PCR) assays for the amplification and detection of pandemic (H1N1) 2009 virus were developed, and their performance characteristics were compared with those of other published diagnostic assays. Thirty-nine samples confirmed to be positive for pandemic (H1N1) 2009 virus from Alberta, Canada, and six additional samples that were positive for influenza A virus but that were not typeable by using published seasonal influenza H1/H3 virus assays were available for this validation. Amplification and direct sequencing of the products was considered the "gold standard" for case identification. The new assays were sensitive and able to reproducibly detect virus in a 10(-6) dilution of 4 x 10(6) 50% tissue culture infective doses/ml when 5 microl was used as the template. They showed 100% specificity and did not cross-react with other respiratory viruses or seasonal influenza A virus subtypes. The coefficient of variation in crossing cycle threshold values for the detection of different template concentrations of pandemic (H1N1) 2009 virus was < or =3.13%, showing good reproducibility. The assays had a wide dynamic range for the detection of pandemic (H1N1) 2009 virus and utilized testing platforms appropriate for high diagnostic throughput with rapid turnaround times. We developed and validated these real-time PCR procedures with the goal that they will be useful for diagnosis and surveillance of pandemic (H1N1) 2009 virus. These findings will contribute to the informed management of this novel virus.
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