ArticleJournal of clinical microbiology2009
Comparison of the EntericBio multiplex PCR system with routine culture for detection of bacterial enteric pathogens.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Diagnostic Accuracy of Multiplex NAAT/PCR and Culture AgainstPathogens (Basel, Switzerland) · 2025Pooled it
- Diagnostic accuracy of multiplex nucleic acid amplification tests forPathogens and global health · 2023Pooled it
- QIAstat-Dx Syndromic Molecular Testing Versus Conventional Diagnostics in Acute Gastroenteritis: Impact on Pathogen Detection and Laboratory Workflow.Microorganisms · 2026Article
- Clinical performance evaluation of TAQPATH Enteric Bacterial Select Panel for the detection of common enteric bacterial pathogens in comparison to routine stool culture and other qPCR-based diagnostic tests.Microbiology spectrum · 2024Article
- Impact of gastrointestinal polymerase chain reaction panels on antibiotic utilization in hospitalized adult patients.Antimicrobial stewardship & healthcare epidemiology : ASHE · 2023Article
- Application of a Multiplex Polymerase Chain Reaction Test for Diagnosing Bacterial Enteritis in Children in a Real-Life Clinical Setting.Children (Basel, Switzerland) · 2021Article
- Molecular characterization of Shigella species isolated from diarrheal patients in Tehran, Iran: phylogenetic typing and its association with virulence gene profiles and a novel description of Shigella invasion associated locus.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Outcomes and clinical relevance of stool multiplex bacterial polymerase chain reaction in patients with acute diarrhea: single center experience.The Korean journal of internal medicine · 2020Article
- Frequency ofReports of biochemistry & molecular biology · 2019Article
- A sensitive gold-nanorods-based nanobiosensor for specific detection of Campylobacter jejuni and Campylobacter coli.Journal of nanobiotechnology · 2019Article
- PhasomeIt: an 'omics' approach to cataloguing the potential breadth of phase variation in the genus Campylobacter.Microbial genomics · 2018Article
- Changing Diagnostic Methods and Increased Detection of Verotoxigenic Escherichia coli, Ireland.Emerging infectious diseases · 2016Article
- Application of a multiplex PCR assay for the detection of gastrointestinal pathogens in a rural African setting.BMC infectious diseases · 2016Article
- Clinical Significance of Fecal Lactoferrin and Multiplex Polymerase Chain Reaction in Patients with Acute Diarrhea.Gut and liver · 2015Article
- Multicenter evaluation of the BD max enteric bacterial panel PCR assay for rapid detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga toxin 1 and 2 genes.Journal of clinical microbiology · 2015Article
- A multiplex PCR/LDR assay for simultaneous detection and identification of the NIAID category B bacterial food and water-borne pathogens.Diagnostic microbiology and infectious disease · 2014Article
- Campylobacter ureolyticus: a portrait of the pathogen.Virulence · 2014Review
- An overview of calf diarrhea - infectious etiology, diagnosis, and intervention.Journal of veterinary science · 2014Review
- Simultaneous detection of viral and bacterial enteric pathogens using the Seeplex® Diarrhea ACE detection system.Epidemiology and infection · 2013Article
- Improved detection of bacterial pathogens in patients presenting with gastroenteritis by use of the EntericBio real-time Gastro Panel I assay.Journal of clinical microbiology · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The EntericBio system uses a multiplex PCR assay for the simultaneous detection of Campylobacter spp., Salmonella enterica, Shigella spp., and Escherichia coli O157 from feces. It combines overnight broth enrichment with PCR amplification and detection by hybridization. An evaluation of this system was conducted by comparing the results obtained with the system with those obtained by routine culture, supplemented with alternative PCR detection methods. In a study of 773 samples, routine culture and the EntericBio system yielded 94.6 and 92.4% negative results, respectively. Forty-two samples had positive results by culture, and all of these were positive with the EntericBio system. This system detected an additional 17 positive samples (Campylobacter spp., n = 12; Shigella spp., n = 1; E. coli O157, n = 4), but the results for 5 samples (Campylobacter spp., n = 2; Shigella spp., n = 1; E. coli O157, n = 2) could not be confirmed. The target for Shigella spp. detected by the EntericBio system is the ipaH gene, and the molecular indication of the presence of Shigella spp. was investigated by sequence analysis, which confirmed that the ipaH gene was present in a Klebsiella pneumoniae isolate from the patient. The sensitivity, specificity, positive predictive value, and negative predictive value were 100%, 99.3%, 91.5%, and 100%, respectively. Turnaround times were significantly reduced with the EntericBio system, and a result was available between 24 and 32 h after receipt of the sample in the laboratory. In addition, the amount of laboratory waste was significantly reduced by use of this system. In summary, the EntericBio system proved convenient to use, more sensitive than the conventional culture used in this study, and highly specific; and it generated results significantly faster than routine culture for the pathogens tested.
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