ArticlePlant physiology2009
Dual roles of reactive oxygen species and NADPH oxidase RBOHD in an Arabidopsis-Alternaria pathosystem.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed, 210 citations in OpenAlex.
- Reactive oxygen species production and signal transduction in response to stress triggered by Alternaria elicitors in Brassicaceae.Plant molecular biology · 2025Review
- Suppressing Symptomless Nonhost Resistance of Barley toPlants (Basel, Switzerland) · 2025Article
- Article
- Identification of VcRBOH genes in blueberry and functional characterization of VcRBOHF in plant defense.BMC genomics · 2025Article
- Abscisic acid signaling gates salt-induced responses of plant roots.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cu/Zn superoxide dismutase homologs participate inFrontiers in microbiology · 2025Article
- The OXIDATIVE SIGNAL-INDUCIBLE1 kinase regulates plant immunity by linking microbial pattern-induced reactive oxygen species burst to MAP kinase activation.The Plant cell · 2024Article
- Article
- Glutathione Contribution in Interactions betweenInternational journal of molecular sciences · 2023Article
- Recent advances on genome-wide association studies (GWAS) and genomic selection (GS); prospects for Fusarium head blight research in Durum wheat.Molecular biology reports · 2023Review
- Casparian strips prevent apoplastic diffusion of boric acid into root steles for excess B tolerance.Frontiers in plant science · 2023Article
- The cytosolic thiol peroxidase PRXIIB is an intracellular sensor for HNature plants · 2022Article
- Secreted Glycosyltransferase RsIA_GT ofPathogens (Basel, Switzerland) · 2022Article
- Intertwined Roles of Reactive Oxygen Species and Salicylic Acid Signaling Are Crucial for the Plant Response to Biotic Stress.International journal of molecular sciences · 2022Review
- Sorbitol Reduces Sensitivity toGenes · 2022Article
- Comparative Transcriptome Analysis Unravels Defense Pathways ofFrontiers in plant science · 2022Article
- The Respiratory Burst Oxidase Homolog Protein D (International journal of molecular sciences · 2021Article
- Glutathione contributes to resistance responses to TMV through a differential modulation of salicylic acid and reactive oxygen species.Molecular plant pathology · 2021Article
- Glutathione Metabolism in Plants under Stress: Beyond Reactive Oxygen Species Detoxification.Metabolites · 2021Review
- Electrical Signals, Plant Tolerance to Actions of Stressors, and Programmed Cell Death: Is Interaction Possible?Plants (Basel, Switzerland) · 2021Review
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis (Arabidopsis thaliana) NADPH oxidases have been reported to suppress the spread of pathogen- and salicylic acid-induced cell death. Here, we present dual roles of RBOHD (for respiratory burst oxidase homolog D) in an Arabidopsis-Alternaria pathosystem, suggesting either initiation or prevention of cell death dependent on the distance from pathogen attack. Our data demonstrate that a rbohD knockout mutant exhibits increased spread of cell death at the macroscopic level upon inoculation with the fungus Alternaria brassicicola. However, the cellular patterns of reactive oxygen species accumulation and cell death are fundamentally different in the AtrbohD mutant compared with the wild type. Functional RBOHD causes marked extracellular hydrogen peroxide accumulation as well as cell death in distinct, single cells of A. brassicicola-infected wild-type plants. This single cell response is missing in the AtrbohD mutant, where infection triggers spreading-type necrosis preceded by less distinct chloroplastic hydrogen peroxide accumulation in large clusters of cells. While the salicylic acid analog benzothiadiazole induces the action of RBOHD and the development of cell death in infected tissues, the ethylene inhibitor aminoethoxyvinylglycine inhibits cell death, indicating that both salicylic acid and ethylene positively regulate RBOHD and cell death. Moreover, A. brassicicola-infected AtrbohD plants hyperaccumulate ethylene and free salicylic acid compared with the wild type, suggesting negative feedback regulation of salicylic acid and ethylene by RBOHD. We propose that functional RBOHD triggers death in cells that are damaged by fungal infection but simultaneously inhibits death in neighboring cells through the suppression of free salicylic acid and ethylene levels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.