ArticlePlant physiology2009
Identification of the endodermal vacuole as the iron storage compartment in the Arabidopsis embryo.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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Who cites it
104 citing papers in PubMed, 232 citations in OpenAlex.
- Mapping Labile FeMethods in molecular biology (Clifton, N.J.) · 2027Article
- An indole dimer antifungal metabolite from a rice seed endophyte inhibits ergosterol biosynthesis in fungal pathogens.Applied and environmental microbiology · 2026Article
- Exploring the evolution of the histone deacetylase 2 (HD2) gene family in plants and the role of AtHDT4 in cadmium stress response in Arabidopsis thaliana.Plant cell reports · 2026Article
- Establishment of a Non-transgenic Iron-Biofortified Rice Line Using a Novel HRZ1 Mutation.Rice (New York, N.Y.) · 2026Article
- Abscisic acid regulates iron accumulation in plant seeds via the PYLs-bHLH IVc-YSLs module.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Maintaining sulfur supply to the symbiosome delays nodule senescence in soybean.Nature communications · 2025Article
- Single-cell multiome reveals root hair-specific responses to salt stress.The New phytologist · 2025Article
- Ferroptosis in plant immunity.Plant communications · 2025Review
- Physiological and transcriptional analyses of Arabidopsis primary root growth in response to phosphate starvation under light and dark conditions.Frontiers in plant science · 2025Article
- Untangling iron threads: A deep dive into plant intracellular pools.Quantitative plant biology · 2025Review
- Inorganic nitrogen inhibits symbiotic nitrogen fixation through blocking NRAMP2-mediated iron delivery in soybean nodules.Nature communications · 2024Article
- Imaging of labile Fe2+ and Fe3+ in living Arabidopsis thaliana roots.Plant physiology · 2024Article
- Ferric reduction by a CYBDOM protein counteracts increased iron availability in root meristems induced by phosphorus deficiency.Nature communications · 2024Article
- Human umbilical cord mesenchymal stem cell-derived exosome suppresses programmed cell death in traumatic brain injury via PINK1/Parkin-mediated mitophagy.CNS neuroscience & therapeutics · 2023Article
- Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots.Plant physiology · 2023Article
- GmABCG5, an ATP-binding cassette G transporter gene, is involved in the iron deficiency response in soybean.Frontiers in plant science · 2023Article
- Iron uptake of etioplasts is independent from photosynthesis but applies the reduction-based strategy.Frontiers in plant science · 2023Article
- Black sheep, dark horses, and colorful dogs: a review on the current state of the Gene Ontology with respect to iron homeostasis inFrontiers in plant science · 2023Review
- Perls/DAB Staining to Examine Iron Distribution in Arabidopsis Embryos.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Transcriptomic Analysis of Distal Parts of Roots Reveals Potentially Important Mechanisms Contributing to Limited Flooding Tolerance of Canola (International journal of molecular sciences · 2022Article
44 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Deciphering how cellular iron (Fe) pools are formed, where they are localized, and which ones are remobilized represents an important challenge to better understand Fe homeostasis. The recent development of imaging techniques, adapted to plants, has helped gain insight into these events. We have analyzed the localization of Fe during embryo development in Arabidopsis (Arabidopsis thaliana) with an improved histochemical staining based on Perls coloration intensified by a second reaction with diaminobenzidine and hydrogen peroxide. The procedure, quick to set up and specific for Fe, was applied directly on histological sections, which dramatically increased its subcellular resolution. We have thus unambiguously shown that in dry seeds Fe is primarily stored in the endodermis cell layer, within the vacuoles, from which it is remobilized during germination. In the vit1-1 mutant, in which the Fe pattern is disturbed, Fe is stored in vacuoles of cortex cells of the hypocotyl/radicle axis and in a single subepidermal cell layer in the cotyledons. During the early stages of embryo development, Fe is evenly distributed in the cells of both wild-type and vit1-1 mutants. Fe eventually accumulates in endodermal cells as the vascular system develops, a process that is impaired in vit1-1. Our results have uncovered a new role for the endodermis in Fe storage in the embryo and have established that the Perls/diaminobenzidine staining is a method of choice to detect Fe in plant tissues and cells.
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