ArticleMolecular biology of the cell2009
Kei1: a novel subunit of inositolphosphorylceramide synthase, essential for its enzyme activity and Golgi localization.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Molecular insights into fungal inositol phosphorylceramide synthesis and its inhibition by antifungal aureobasidin A.Nature communications · 2026Article
- Mechanisms of aureobasidin A inhibition and drug resistance in a fungal IPC synthase complex.Nature communications · 2025Article
- Biological Importance of Complex Sphingolipids and Their Structural Diversity in Budding YeastInternational journal of molecular sciences · 2024Review
- Complex sphingolipid profiling and identification of an inositol-phosphorylceramide synthase iniScience · 2024Article
- Article
- Impaired biosynthesis of ergosterol confers resistance to complex sphingolipid biosynthesis inhibitor aureobasidin A in a PDR16-dependent manner.Scientific reports · 2023Article
- The ER calcium channel Csg2 integrates sphingolipid metabolism with autophagy.Nature communications · 2023Article
- The ERAD system is restricted by elevated ceramides.Science advances · 2023Article
- Review
- Flavonoids as Potential Drugs forGenes · 2020Article
- Proper regulation of inositolphosphorylceramide levels is required for acquirement of low pH resistance in budding yeast.Scientific reports · 2020Article
- Lipid Rafts, Sphingolipids, and Ergosterol in Yeast Vacuole Fusion and Maturation.Frontiers in cell and developmental biology · 2020Review
- Auxin-Inducible Depletion of the Essentialome Suggests Inhibition of TORC1 by Auxins and Inhibition of Vrg4 by SDZ 90-215, a Natural Antifungal Cyclopeptide.G3 (Bethesda, Md.) · 2019Article
- Sphingolipid accumulation causes mitochondrial dysregulation and cell death.Cell death and differentiation · 2017Article
- Membrane engineering of S. cerevisiae targeting sphingolipid metabolism.Scientific reports · 2017Article
- Control of Plasma Membrane Permeability by ABC Transporters.Eukaryotic cell · 2015Article
- The yeast sphingolipid signaling landscape.Chemistry and physics of lipids · 2014Review
- Orm proteins integrate multiple signals to maintain sphingolipid homeostasis.The Journal of biological chemistry · 2013Article
- Action of multiple endoplasmic reticulum chaperon-like proteins is required for proper folding and polarized localization of Kre6 protein essential in yeast cell wall β-1,6-glucan synthesis.The Journal of biological chemistry · 2012Article
- Kre6 protein essential for yeast cell wall beta-1,6-glucan synthesis accumulates at sites of polarized growth.The Journal of biological chemistry · 2011Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal sphingolipids have inositol-phosphate head groups, which are essential for the viability of cells. These head groups are added by inositol phosphorylceramide (IPC) synthase, and AUR1 has been thought to encode this enzyme. Here, we show that an essential protein encoded by KEI1 is a novel subunit of IPC synthase of Saccharomyces cerevisiae. We find that Kei1 is localized in the medial-Golgi and that Kei1 is cleaved by Kex2, a late Golgi processing endopeptidase; therefore, it recycles between the medial- and late Golgi compartments. The growth defect of kei1-1, a temperature-sensitive mutant, is effectively suppressed by the overexpression of AUR1, and Aur1 and Kei1 proteins form a complex in vivo. The kei1-1 mutant is hypersensitive to aureobasidin A, a specific inhibitor of IPC synthesis, and the IPC synthase activity in the mutant membranes is thermolabile. A part of Aur1 is missorted to the vacuole in kei1-1 cells. We show that the amino acid substitution in kei1-1 causes release of Kei1 during immunoprecipitation of Aur1 and that Aur1 without Kei1 has hardly detectable IPC synthase activity. From these results, we conclude that Kei1 is essential for both the activity and the Golgi localization of IPC synthase.
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