ReviewJournal of chromatography. A2009
Methods for proteomic analysis of transcription factors.
Review in Journal of chromatography. A, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- Molecular mechanism of ZmWRKY36 mediated maize resistance to Bipolaris maydis.BMC plant biology · 2026Article
- Alterations of the skeletal muscle nuclear proteome after acute exercise reveals a posttranscriptional influence.American journal of physiology. Cell physiology · 2025Article
- Unraveling biomolecular interactions: a comprehensive review of the electromobility shift assay.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Review
- Systems Biology for Drug Target Discovery in Acute Myeloid Leukemia.International journal of molecular sciences · 2024Article
- Pan-cancer quantitation of epithelial-mesenchymal transition dynamics using parallel reaction monitoring-based targeted proteomics approach.Journal of translational medicine · 2022Article
- Transcription Factor Movement and Exercise-Induced Mitochondrial Biogenesis in Human Skeletal Muscle: Current Knowledge and Future Perspectives.International journal of molecular sciences · 2022Review
- SUMOylated non-canonical polycomb PRC1.6 complex as a prerequisite for recruitment of transcription factor RBPJ.Epigenetics & chromatin · 2021Article
- Covalent Modification of Proteins by Plant-Derived Natural Products: Proteomic Approaches and Biological Impacts.Proteomics · 2021Review
- Identifying nucleic acid-associated proteins in Mycobacterium smegmatis by mass spectrometry-based proteomics.BMC molecular and cell biology · 2020Article
- A sensitive and simple targeted proteomics approach to quantify transcription factor and membrane proteins of the unfolded protein response pathway in glioblastoma cells.Scientific reports · 2019Article
- Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes.Antimicrobial agents and chemotherapy · 2018Article
- The human DNA ends proteome uncovers an unexpected entanglement of functional pathways.Nucleic acids research · 2016Article
- T(3): targeted proteomics of DNA-binding proteins.Analytical biochemistry · 2015Article
- Gel-based proteomics in plants: time to move on from the tradition.Frontiers in plant science · 2015Article
- Pharmacological manipulation of transcription factor protein-protein interactions: opportunities and obstacles.Cell regeneration (London, England) · 2015Article
- Affinity purification strategies for proteomic analysis of transcription factor complexes.Journal of proteome research · 2013Article
- Investigation of stable and transient protein-protein interactions: Past, present, and future.Proteomics · 2013Review
- DNA-protein interactions: methods for detection and analysis.Molecular and cellular biochemistry · 2012Review
- TGF-β signaling via Smad4 drives IL-10 production in effector Th1 cells and reduces T-cell trafficking in EAE.European journal of immunology · 2011Article
- Global proteomics analysis of testis and ovary in adult zebrafish (Danio rerio).Fish physiology and biochemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Investigation of the transcription factor (TF) proteome presents challenges including the large number of low abundance and post-translationally modified proteins involved. Specialized purification and analysis methods have been developed over the last decades which facilitate the study of the TF proteome and these are reviewed here. Generally applicable proteomics methods that have been successfully applied are also discussed. TFs are selectively purified by affinity techniques using the DNA response element (RE) as the basis for highly specific binding, and several agents have been discovered that either enhance binding or diminish non-specific binding. One such affinity method called "trapping" enables purification of TFs bound to nM concentrations and recovery of TF complexes in a highly purified state. The electrophoretic mobility shift assay (EMSA) is the most important assay of TFs because it provides both measures of the affinity and amount of the TF present. Southwestern (SW) blotting and DNA-protein crosslinking (DPC) allow in vitro estimates of DNA-binding-protein mass, while chromatin immunoprecipitation (ChIP) allows confirmation of promoter binding in vivo. Two-dimensional gel electrophoresis methods (2-DE), and 3-DE methods which combines EMSA with 2-DE, allow further resolution of TFs. The synergy of highly selective purification and analytical strategies has led to an explosion of knowledge about the TF proteome and the proteomes of other DNA- and RNA-binding proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.