ArticleParticle and fibre toxicology2009
Genotoxicity of nano/microparticles in in vitro micronuclei, in vivo comet and mutation assay systems.
Article in Particle and fibre toxicology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 101 citations in OpenAlex.
- A Regulatory-Compliant Genotoxicity Study of a Mixture of C60 and C70 Fullerenes Dissolved in Olive Oil Using the Mammalian Micronucleus Test.Nanomaterials (Basel, Switzerland) · 2025Article
- Cell Death and Proliferation Variability Caused by Different Dust Clay Minerals Using the Single-Cell Method.GeoHealth · 2025Article
- An In Vitro Model to Assess Early Immune Markers Following Co-Exposure of Epithelial Cells to Carbon Black (Nano)Particles in the Presence ofBiomedicines · 2024Article
- Recent Advances in Kaolinite Nanoclay as Drug Carrier for Bioapplications: A Review.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- In vivo genotoxicity assessment of a multiwalled carbon nanotube in a mouse ex vivo culture.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2022Article
- In Vitro Genotoxicity Evaluation of an Antiseptic Formulation Containing Kaolin and Silver Nanoparticles.Nanomaterials (Basel, Switzerland) · 2022Article
- The Puzzling Potential of Carbon Nanomaterials: General Properties, Application, and Toxicity.Nanomaterials (Basel, Switzerland) · 2020Review
- Genotoxicity of micro- and nano-particles of kaolin in human primary dermal keratinocytes and fibroblasts.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2020Article
- Toxicity of Zero- and One-Dimensional Carbon Nanomaterials.Nanomaterials (Basel, Switzerland) · 2019Review
- Establishment of an in vivo simulating co-culture assay platform for genotoxicity of multi-walled carbon nanotubes.Cancer science · 2018Article
- Cobalt nanoparticles induce lung injury, DNA damage and mutations in mice.Particle and fibre toxicology · 2017Article
- Effect of physicochemical character differences on the genotoxic potency of kaolin.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2017Article
- Absence ofGenes and environment : the official journal of the Japanese Environmental Mutagen Society · 2017Article
- A perspective on the potential risks of emerging contaminants to human and environmental health.Environmental science and pollution research international · 2015Review
- [Study on the Relationship between the Inhalable Fine Particulate Matter of Xuanwei Coal Combustion and Lung Cancer].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2015Article
- DNA damage following pulmonary exposure by instillation to low doses of carbon black (Printex 90) nanoparticles in mice.Environmental and molecular mutagenesis · 2015Article
- Magnetite Nanoparticles Induce Genotoxicity in the Lungs of Mice via Inflammatory Response.Nanomaterials (Basel, Switzerland) · 2014Article
- Intraperitoneal exposure to nano/microparticles of fullerene (C₆₀) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (Danio rerio) brain.BioMed research international · 2013Article
- Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice: effects on liver DNA strand breaks in dams and offspring.Nanotoxicology · 2012Article
- Quantification of particle-induced inflammatory stress response: a novel approach for toxicity testing of earth materials.Geochemical transactions · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecently, manufactured nano/microparticles such as fullerenes (C60), carbon black (CB) and ceramic fiber are being widely used because of their desirable properties in industrial, medical and cosmetic fields. However, there are few data on these particles in mammalian mutagenesis and carcinogenesis. To examine genotoxic effects by C60, CB and kaolin, an in vitro micronuclei (MN) test was conducted with human lung cancer cell line, A549 cells. In addition, DNA damage and mutations were analyzed by in vivo assay systems using male C57BL/6J or gpt delta transgenic mice which were intratracheally instilled with single or multiple doses of 0.2 mg per animal of particles.
resultsIn in vitro genotoxic analysis, increased MN frequencies were observed in A549 cells treated with C60, CB and kaolin in a dose-dependent manner. These three nano/microparticles also induced DNA damage in the lungs of C57BL/6J mice measured by comet assay. Moreover, single or multiple instillations of C60 and kaolin, increased either or both of gpt and Spi- mutant frequencies in the lungs of gpt delta transgenic mice. Mutation spectra analysis showed transversions were predominant, and more than 60% of the base substitutions occurred at G:C base pairs in the gpt genes. The G:C to C:G transversion was commonly increased by these particle instillations.
conclusionManufactured nano/microparticles, CB, C60 and kaolin, were shown to be genotoxic in in vitro and in vivo assay systems.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.