ArticleStem cells (Dayton, Ohio)2009
Developmental regulation of human embryonic stem cell-derived neurons by calcium entry via transient receptor potential channels.
Article in Stem cells (Dayton, Ohio), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Endogenous ATP-powered nanomotors directing neural stem cell differentiation for Parkinson's disease treatment.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Aging aggravated liver ischemia and reperfusion injury by promoting oxidized mtDNA mediated-macrophage pyroptosis through acetylated MCU-dependent calcium uptake.Cell death discovery · 2025Article
- TRP Channels in Stroke.Neuroscience bulletin · 2024Review
- Molecular Components of Store-Operated Calcium Channels in the Regulation of Neural Stem Cell Physiology, Neurogenesis, and the Pathology of Huntington's Disease.Frontiers in cell and developmental biology · 2021Review
- Schistosome TRP channels: An appraisal.International journal for parasitology. Drugs and drug resistance · 2020Review
- How TRPC Channels Modulate Hippocampal Function.International journal of molecular sciences · 2020Review
- Default Patterning Produces Pan-cortical Glutamatergic and CGE/LGE-like GABAergic Neurons from Human Pluripotent Stem Cells.Stem cell reports · 2017Article
- Elevated Expression of TRPC4 in Cortical Lesions of Focal Cortical Dysplasia II and Tuberous Sclerosis Complex.Journal of molecular neuroscience : MN · 2017Article
- Flow Shear Stress Enhances the Proliferative Potential of Cultured Radial Glial Cells Possibly Via an Activation of Mechanosensitive Calcium Channel.Experimental neurobiology · 2017Article
- Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells.Stem cells international · 2017Article
- Functional properties of in vitro excitatory cortical neurons derived from human pluripotent stem cells.The Journal of physiology · 2016Review
- Critical roles of Gi/o proteins and phospholipase C-δ1 in the activation of receptor-operated TRPC4 channels.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Functional Properties of Human Stem Cell-Derived Neurons in Health and Disease.Stem cells international · 2016Review
- Transient receptor potential channels and their role in modulating radial glial-neuronal interaction: a signaling pathway involving mGluR5.Stem cells and development · 2015Article
- Canonical transient receptor potential 4 and its small molecule modulators.Science China. Life sciences · 2015Review
- Ion channels in regulation of neuronal regenerative activities.Translational stroke research · 2014Review
- Substrate-dependent gene regulation of self-assembled human MSC spheroids on chitosan membranes.BMC genomics · 2014Article
- Plasticity of calcium signaling cascades in human embryonic stem cell-derived neural precursors.Stem cells and development · 2013Article
- Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny.Stem cells international · 2013Article
- Platelet-derived growth factor-BB restores human immunodeficiency virus Tat-cocaine-mediated impairment of neurogenesis: role of TRPC1 channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Spontaneous calcium (Ca(2+)) transients in the developing nervous system can affect proliferation, migration, neuronal subtype specification, and neurite outgrowth. Here, we show that telencephalic human neuroepithelia (hNE) and postmitotic neurons (PMNs) generated from embryonic stem cells display robust Ca(2+) transients. Unlike previous reports in animal models, transients occurred by a Gd(3+)/La(3+)-sensitive, but thapsigargin- and Cd(2+)-insensitive, mechanism, strongly suggestive of a role for transient receptor potential (Trp) channels. Furthermore, Ca(2+) transients in PMNs exhibited an additional sensitivity to the canonical Trp (TrpC) antagonist SKF96365 and shRNA-mediated knockdown of the TrpC1 subunit. Functionally, inhibition of Ca(2+) transients in dividing hNE cells led to a significant reduction in proliferation, whereas either pharmacological inhibition or shRNA-mediated knockdown of the TrpC1 and TrpC4 subunits significantly reduced neurite extension in PMNs. Primary neurons cultured from fetal human cortex displayed nearly identical Ca(2+) transients and pharmacological sensitivities to Trp channel antagonists. Together these data suggest that Trp channels present a novel mechanism for controlling Ca(2+) transients in human neurons and may offer a target for regulating proliferation and neurite outgrowth when engineering cells for therapeutic transplantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.