ArticleInternational journal of oncology2009
Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis.
Article in International journal of oncology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
- Astrocyte involvement in brain metastasis: from biological mechanisms to therapeutic strategies.Cancer metastasis reviews · 2025Review
- Brain microenvironment orchestrates highly aggressive tumor variants: current trends and therapeutic approaches.Frontiers in aging neuroscience · 2025Review
- Development of rhesus macaque astrocyte cell lines supporting infection with a panel of viruses.PloS one · 2024Article
- The microenvironment of brain metastases from solid tumors.Neuro-oncology advances · 2021Article
- Astrocytes and microglia in neurodegenerative diseases: Lessons from human in vitro models.Progress in neurobiology · 2021Review
- Generation of an immortalized astrocytic cell line fromHeliyon · 2021Article
- The biology and therapeutic management of melanoma brain metastases.Biochemical pharmacology · 2018Review
- Reactive Astrocytes in Brain Metastasis.Frontiers in oncology · 2017Review
- Treatment of experimental human breast cancer and lung cancer brain metastases in mice by macitentan, a dual antagonist of endothelin receptors, combined with paclitaxel.Neuro-oncology · 2016Article
- Intercellular transfer of small RNAs from astrocytes to lung tumor cells induces resistance to chemotherapy.Oncotarget · 2016Article
- Macitentan, a Dual Endothelin Receptor Antagonist, in Combination with Temozolomide Leads to Glioblastoma Regression and Long-term Survival in Mice.Clinical cancer research : an official journal of the American Association for Cancer Research · 2015Article
- Role of the endothelin axis in astrocyte- and endothelial cell-mediated chemoprotection of cancer cells.Neuro-oncology · 2014Article
- MicroRNAs in brain metastases: potential role as diagnostics and therapeutics.International journal of molecular sciences · 2014Review
- Apolipoprotein E Mimetic Promotes Functional and Histological Recovery in Lysolecithin-Induced Spinal Cord Demyelination in Mice.Journal of neurology & neurophysiology · 2013Article
- Induction of cytokines and growth factors by rapamycin in the microenvironment of brain metastases of lung cancer.Oncology letters · 2013Article
- Reactive astrocytes promote the metastatic growth of breast cancer stem-like cells by activating Notch signalling in brain.EMBO molecular medicine · 2013Article
- Article
- The role of astrocytes in CNS tumors: pre-clinical models and novel imaging approaches.Frontiers in cellular neuroscience · 2013Article
- The brain microenvironment negatively regulates miRNA-768-3p to promote K-ras expression and lung cancer metastasis.Scientific reports · 2013Article
- Expression of EAAT2 in neurons and protoplasmic astrocytes during human cortical development.The Journal of comparative neurology · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Astrocytes play a critical role in maintaining cerebral homeostasis and their dysregulation is thought to contribute to the pathogenesis of several diseases, including brain cancer and metastasis. Similar to the human disease, we found that lung and melanoma metastases in the mouse brain are accompanied by a reactive gliosis. To begin to study the biology of astrocytes and examine how these cells might contribute to metastasis formation and progression in the brain, we generated a conditionally immortal astrocyte cell line from H-2Kb-tsA58 mice. Astrocytes grown in culture expressed glial fibrillary acid protein (GFAP), glutamate receptor 1, and the N-methyl-D-aspartate (NMDA) receptor. Astrocytes also expressed the glial-specific transporters excitatory amino acid transporter 1 (EAAT1) and EAAT2. Astrocytes grown under permissive conditions (33 degrees C) expressed SV40 large T antigen and had a doubling time of 36 h, whereas expression of SV40 large T antigen was negligible in astrocytes grown at 37 degrees C for 72 h, which coincided with a plateau in cell division. In a co-culture assay with human lung adenocarcinoma cells (PC14-PE6), astrocytes activated programs in the tumor cells that signal for cell division and survival. Hence, the immortalized cell line will be useful for studying the role of astrocytes in disease processes in the brain, such as metastasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.