ArticleThe Korean journal of parasitology2009
Comparative sensitivity of PCR primer sets for detection of Cryptosporidium parvum.
Article in The Korean journal of parasitology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Evaluating the role of synanthropic filth flies in the transmission of zoonotic parasites: field and laboratory evidence from different animal rearing sites in upper Egypt with focus on Cryptosporidium spp.BMC veterinary research · 2025Article
- Comparative evaluation of various diagnostic techniques for detection ofIranian journal of veterinary research · 2022Article
- Detection and Molecular Identification of Cryptosporidium Species Among Children with Malignancies.Acta parasitologica · 2021Article
- Determination of the Microbial and Chemical Loads in Rivers from the Quito Capital Province of Ecuador (Pichincha)-A Preliminary Analysis of Microbial and Chemical Quality of the Main Rivers.International journal of environmental research and public health · 2020Article
- Prevalence and molecular characterization ofOne health (Amsterdam, Netherlands) · 2020Article
- Molecular characterization of Cryptosporidium isolates from diarrheal dairy calves in France.Veterinary parasitology, regional studies and reports · 2019Article
- Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016.The Korean journal of parasitology · 2019Article
- Monitoring of Noxious Protozoa for Management of Natural Water Resources.The Korean journal of parasitology · 2018Article
- Multiplex-Touchdown PCR to Simultaneously DetectThe Korean journal of parasitology · 2016Article
- Molecular detection and characterization of Cryptosporidium spp. among breeding cattery cats in Japan.Parasitology research · 2016Article
- Cryptosporidium species and subtype analysis in diarrhoeic pre-weaned lambs and goat kids from north-western Spain.Parasitology research · 2015Article
- A comparison of nested PCR assay with conventional techniques for diagnosis of intestinal cryptosporidiosis in AIDS cases from northern India.Journal of parasitology research · 2014Article
- Evaluation of an immunochromatographic dip strip test for simultaneous detection of Cryptosporidium spp, Giardia duodenalis, and Entamoeba histolytica antigens in human faecal samples.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Epidemiology and molecular relationships of Cryptosporidium spp. in people, primates, and livestock from Western Uganda.PLoS neglected tropical diseases · 2012Article
- Detection of Cryptosporidium parvum and Cryptosporidium hominis in human patients in Cairo, Egypt.Parasitology research · 2012Article
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3 authors.
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Abstract
Improved methods for detection of Cryptosporidium oocysts in environmental and clinical samples are urgently needed to improve detection of cryptosporidiosis. We compared the sensitivity of 7 PCR primer sets for detection of Cryptosporidium parvum. Each target gene was amplified by PCR or nested PCR with serially diluted DNA extracted from purified C. parvum oocysts. The target genes included Cryptosporidium oocyst wall protein (COWP), small subunit ribosomal RNA (SSU rRNA), and random amplified polymorphic DNA. The detection limit of the PCR method ranged from 10(3) to 10(4) oocysts, and the nested PCR method was able to detect 10(0) to 10(2) oocysts. A second-round amplification of target genes showed that the nested primer set specific for the COWP gene proved to be the most sensitive one compared to the other primer sets tested in this study and would therefore be useful for the detection of C. parvum.
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