ArticleNucleic acids research2009
Real-time DNA microarray analysis.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- AssayBLAST v2: major update improving reliability and reporting of the in silico analysis of molecular multi-parameter assays.BMC bioinformatics · 2026Article
- Deep Learning Predicts Dissimilar DNA-DNA Binding and Engineers Hyperconnected Networks.Nature communications · 2026Article
- Inherent stochasticity, noise and limits of detection in continuous and time-gated fluorescence systems.PloS one · 2024Article
- Surface-modified CMOS biosensors.Frontiers in bioengineering and biotechnology · 2024Review
- The Intestinal Microbiota May Be a Potential Theranostic Tool for Personalized Medicine.Journal of personalized medicine · 2022Review
- Multiplexed identification, quantification and genotyping of infectious agents using a semiconductor biochip.Nature biotechnology · 2018Article
- An array-based melt curve analysis method for the identification and classification of closely related pathogen strains.Biology methods & protocols · 2018Article
- A Fully Integrated CMOS Fluorescence Biochip for DNA and RNA Testing.IEEE journal of solid-state circuits · 2017Article
- Personalised proteome analysis by means of protein microarrays made from individual patient samples.Scientific reports · 2017Article
- PCR-Independent Detection of Bacterial Species-Specific 16S rRNA at 10 fM by a Pore-Blockage Sensor.Biosensors · 2016Article
- Profiling of Selected MicroRNAs in Proliferative Eutopic Endometrium of Women with Ovarian Endometriosis.BioMed research international · 2015Article
- Reference genes for normalizing transcription in diploid and tetraploid Arabidopsis.Scientific reports · 2014Article
- BIOPHYSICAL PROPERTIES OF NUCLEIC ACIDS AT SURFACES RELEVANT TO MICROARRAY PERFORMANCE.Biomaterials science · 2014Article
- Real-time fluorescent image analysis of DNA spot hybridization kinetics to assess microarray spot heterogeneity.Analytical chemistry · 2012Article
- Sequence-specific nucleic acid detection from binary pore conductance measurement.Journal of the American Chemical Society · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a quantification method for affinity-based DNA microarrays which is based on the real-time measurements of hybridization kinetics. This method, i.e. real-time DNA microarrays, enhances the detection dynamic range of conventional systems by being impervious to probe saturation in the capturing spots, washing artifacts, microarray spot-to-spot variations, and other signal amplitude-affecting non-idealities. We demonstrate in both theory and practice that the time-constant of target capturing in microarrays, similar to all affinity-based biosensors, is inversely proportional to the concentration of the target analyte, which we subsequently use as the fundamental parameter to estimate the concentration of the analytes. Furthermore, to empirically validate the capabilities of this method in practical applications, we present a FRET-based assay which enables the real-time detection in gene expression DNA microarrays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.