ArticleNeuroscience2009
Delayed treatments for stroke influence neuronal death in rat organotypic slice cultures subjected to oxygen glucose deprivation.
Article in Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Metformin-dependent variation of microglia phenotype dictates pericytes maturation under oxygen-glucose deprivation.Tissue barriers · 2022Article
- Nanoparticle-microglial interaction in the ischemic brain is modulated by injury duration and treatment.Bioengineering & translational medicine · 2020Article
- Organotypic Brain Slice Culture Microglia Exhibit Molecular Similarity to Acutely-Isolated Adult Microglia and Provide a Platform to Study Neuroinflammation.Frontiers in cellular neuroscience · 2020Article
- Activation of Sigma Receptors With Afobazole Modulates Microglial, but Not Neuronal, Apoptotic Gene Expression in Response to Long-Term Ischemia Exposure.Frontiers in neuroscience · 2019Article
- Article
- Tissue Response to Neural Implants: The Use of Model Systems Toward New Design Solutions of Implantable Microelectrodes.Frontiers in neuroscience · 2019Review
- Microglial Function during Glucose Deprivation: Inflammatory and Neuropsychiatric Implications.Molecular neurobiology · 2018Article
- Mitochondria, Bioenergetics and Excitotoxicity: New Therapeutic Targets in Perinatal Brain Injury.Frontiers in cellular neuroscience · 2017Review
- Effects of Gualou Guizhi Decoction Aqueous Extract on Axonal Regeneration in Organotypic Cortical Slice Culture after Oxygen-Glucose Deprivation.Evidence-based complementary and alternative medicine : eCAM · 2017Article
- Roles of sigma-1 receptors in Alzheimer's disease.International journal of clinical and experimental medicine · 2015Review
- Leukemia inhibitor factor promotes functional recovery and oligodendrocyte survival in rat models of focal ischemia.The European journal of neuroscience · 2014Article
- Monocytes are essential for the neuroprotective effect of human cord blood cells following middle cerebral artery occlusion in rat.Molecular and cellular neurosciences · 2014Article
- The spleen contributes to stroke induced neurodegeneration through interferon gamma signaling.Metabolic brain disease · 2012Article
- High-throughput single-cell manipulation in brain tissue.PloS one · 2012Article
- Human umbilical cord blood stem cells: rational for use as a neuroprotectant in ischemic brain disease.International journal of molecular sciences · 2010Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A major limitation of current stroke therapies is the need to treat candidate patients within 3 h of stroke onset. Human umbilical cord blood cell (HUCBC) and the sigma receptor agonist 1,3, di-o-tolylguanidine (DTG) administration both caused significant reductions in brain damage in the rat middle cerebral artery occlusion model of stroke when administered at delayed timepoints. In vivo, these treatments suppress the infiltration of peripheral lymphocytes into the brain in addition to decreasing neurodegeneration. An ex vivo organotypic slice culture (OTC) model was utilized to characterize the efficacy of these treatments in mitigating neurodegeneration in ischemic brain tissue in the absence of the peripheral immune system. Slice cultures subjected to oxygen glucose deprivation (OGD) had significantly elevated levels of degenerating neurons and microglial nitric oxide production when compared to their normoxic counterparts. In cultures subjected to OGD, HUCBC but not DTG treatment reduced the number of degenerating neurons and the production of microglial derived nitric oxide back to levels detected in normoxic controls. These data show that HUCBC treatment can mediate direct neuroprotection and suppress innate inflammation in ischemic brain tissue in the absence of the peripheral immune system, whereas DTG requires peripheral effects to mediate neuroprotection. These experiments yield insight into the mechanisms by which these neuroprotective treatments function at delayed timepoints following stroke.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.