ArticleProceedings of the National Academy of Sciences of the United States of America1990
Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 136 citations in OpenAlex.
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- Conventional IVF is feasible in preimplantation genetic testing for aneuploidy.Journal of assisted reproduction and genetics · 2023Article
- Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR.Plant methods · 2018Article
- Immuno-PCR technology for detection of natural human antibodies against LeGlycoconjugate journal · 2017Article
- Artifactual mutations resulting from DNA lesions limit detection levels in ultrasensitive sequencing applications.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
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- Identification of FAD2 and FAD3 genes in Brassica napus genome and development of allele-specific markers for high oleic and low linolenic acid contents.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers.BMC biotechnology · 2011Article
- 3'-Protected 2'-deoxynucleoside 5'-triphosphates as a tool for heat-triggered activation of polymerase chain reaction.Analytical chemistry · 2009Article
- Direct molecular haplotyping of long-range genomic DNA with M1-PCR.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Electrophile and oxidant damage of mitochondrial DNA leading to rapid evolution of homoplasmic mutations.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Multiplex loss of heterozygosity analysis by using single or very few cells.The Journal of molecular diagnostics : JMD · 2002Article
- Signal amplification through nucleotide extension and excision on a dendritic DNA platform.Nucleic acids research · 2000Article
- Combined use of biallelic and microsatellite Y-chromosome polymorphisms to infer affinities among African populations.American journal of human genetics · 1999Article
- Rare germinal unequal crossing-over leading to recombinant gene formation and gene duplication in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
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- Evidence for numerous omp1 alleles of porcine Chlamydia trachomatis and novel chlamydial species obtained by PCR.Journal of clinical microbiology · 1997Article
- Detection of Toxoplasma gondii tachyzoites and bradyzoites in blood, urine, and brains of infected mice.Clinical and diagnostic laboratory immunology · 1996Article
- Combinatorial regulation of the Saccharomyces cerevisiae CAR1 (arginase) promoter in response to multiple environmental signals.Molecular and cellular biology · 1996Article
- Multiplex genotype determination at a large number of gene loci.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a procedure that allows the detection of polymerase chain reaction (PCR) products derived from a single target DNA molecule in a human sperm without using radioactive probes. With this method, three genetic loci present in a single sperm can be amplified simultaneously. The amplification procedure is specific as well as efficient and permits detection of the PCR product by ethidium bromide staining after polyacrylamide gel electrophoresis. When allele-specific PCR primers that differ in length are used, the size of the PCR products of different alleles also vary in length, allowing the allelic state at each locus to be determined electrophoretically. Studies on individual sperm by using this procedure should facilitate the measurement of genetic recombination in humans over small physical distances. The ability to directly analyze the allelic state of PCR products from one cell rapidly and simply will also be useful for the prenatal diagnosis of genetic disease, especially in the analysis of single blastomeres taken from in vitro fertilized eggs prior to implantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.