ArticleJournal of clinical microbiology2009
High-level rifampin resistance correlates with multiple mutations in the rpoB gene of pulmonary tuberculosis isolates from the Afghanistan border of Iran.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Efflux pumps positively contribute to rifampin resistance in rpoB mutant Mycobacterium tuberculosis.Annals of clinical microbiology and antimicrobials · 2025Article
- Multi-drug resistance and compensatory mutations in Mycobacterium tuberculosis in Vietnam.Tropical medicine & international health : TM & IH · 2025Article
- Epidemiology of first- and second-line drugs-resistant pulmonary tuberculosis in Iran: Systematic review and meta-analysis.Journal of clinical tuberculosis and other mycobacterial diseases · 2024Review
- Compensatory effects ofEmerging microbes & infections · 2021Article
- Rifampicin-resistant tuberculosis in Iran: A systematic review and meta-analysis.Iranian journal of basic medical sciences · 2021Review
- Antibiotic resistance by high-level intrinsic suppression of a frameshift mutation in an essential gene.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Glutamine Insertion at Codon 432 of RpoB Confers Rifampicin Resistance inFrontiers in microbiology · 2020Article
- Evolution of Rifampin Resistance in Escherichia coli and Mycobacterium smegmatis Due to Substandard Drugs.Antimicrobial agents and chemotherapy · 2019Article
- Insights into the processes that drive the evolution of drug resistance inEvolutionary applications · 2018Review
- Have compensatory mutations facilitated the current epidemic of multidrug-resistant tuberculosis?Emerging microbes & infections · 2018Article
- Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae.Nature communications · 2018Article
- Plasmid-based high-resolution melting analysis for accurate detection of rpoB mutations in Mycobacterium tuberculosis isolates from Moroccan patients.BMC infectious diseases · 2017Article
- Comparative Genome Analysis and Global Phylogeny of the Toxin Variant Clostridium difficile PCR Ribotype 017 Reveals the Evolution of Two Independent Sublineages.Journal of clinical microbiology · 2017Article
- Rifabutin Resistance Associated with Double Mutations inFrontiers in microbiology · 2017Article
- First-Line Anti-Tubercular Drug Resistance of Mycobacterium tuberculosis in IRAN: A Systematic Review.Frontiers in microbiology · 2016Review
- Alarmingly High Segregation Frequencies of Quinolone Resistance Alleles within Human and Animal Microbiomes Are Not Explained by Direct Clinical Antibiotic Exposure.Genome biology and evolution · 2015Article
- Molecular patterns of multidrug resistance ofInternational journal of mycobacteriology · 2013Article
- The rationale for using rifabutin in the treatment of MDR and XDR tuberculosis outbreaks.PloS one · 2013Article
- Mutation analysis of mycobacterial rpoB genes and rifampin resistance using recombinant Mycobacterium smegmatis.Antimicrobial agents and chemotherapy · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to investigate the significance of multiple mutations in the rpoB gene as well as predominant nucleotide changes and their correlation with high levels of resistance to rifampin (rifampicin) in Mycobacterium tuberculosis isolates that were randomly collected from the sputa of 46 patients with primary and secondary cases of active pulmonary tuberculosis from the southern region (Afghanistan border) of Iran where tuberculosis is endemic. Drug susceptibility testing was performed using the CDC standard conventional proportional method. DNA extraction, rpoB gene amplification, and DNA sequencing analysis were performed. Thirty-five (76.09%) isolates were found to have multiple mutations (two to four) in the rpoB (beta-subunit) gene. Furthermore, we demonstrate that the combination of mutations with more prevalent nucleotide changes were observed in codons 523, 526, and 531, indicating higher frequencies of mutations among patients with secondary infection. In this study, 76.08% (n = 35) of all isolates found to have mutation combinations involving nucleotide changes in codons 523 (GGG-->GCG), 531 (TCG-->TTG or TTC), and 526 (CAC-->CGC, TTC, AAC, or CAA) demonstrated an association with higher levels of resistance to rifampin (MIC, >or=100 microg/ml).
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