ArticleThe Journal of general physiology2009
Temperature dependence of proton permeation through a voltage-gated proton channel.
Article in The Journal of general physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 55 citations in OpenAlex.
- Functional evidence of Hv1-mediated proton flux in cardiac mitochondria.Channels (Austin, Tex.) · 2026Article
- The Role of Ion-Transporting Proteins on Crosstalk Between the Skeletal Muscle and Central Nervous Systems Elicited by Physical Exercise.Molecular neurobiology · 2025Review
- Asymmetric Lipid Bilayers and Potassium Channels Embedded Therein in the Contact Bubble Bilayer.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Temperature Dependent Activity of the Voltage-Gated Proton Channel.Advances in experimental medicine and biology · 2024Review
- Role of the Voltage-Gated Proton Channel Hv1 in Nervous Systems.Neuroscience bulletin · 2023Review
- The voltage-gated proton channel hHv1 is functionally expressed in human chorion-derived mesenchymal stem cells.Scientific reports · 2020Article
- Scaling Behavior of Ionic Transport in Membrane Nanochannels.Nano letters · 2018Article
- The Role of Proton Transport in Gating Current in a Voltage Gated Ion Channel, as Shown by Quantum Calculations.Sensors (Basel, Switzerland) · 2018Article
- HistidineThe Journal of general physiology · 2018Article
- Cooperative electrogenic proton transport pathways in the plasma membrane of the proton-secreting osteoclast.Pflugers Archiv : European journal of physiology · 2018Review
- CrossTalk proposal: Proton permeation through HThe Journal of physiology · 2017Article
- Extracellular phosphates enhance activities of voltage-gated proton channels and production of reactive oxygen species in murine osteoclast-like cells.Pflugers Archiv : European journal of physiology · 2017Article
- Nicotine inhibits activation of microglial proton currents via interactions with α7 acetylcholine receptors.The journal of physiological sciences : JPS · 2017Article
- The Hv1 proton channel responds to mechanical stimuli.The Journal of general physiology · 2016Article
- The intimate and controversial relationship between voltage-gated proton channels and the phagocyte NADPH oxidase.Immunological reviews · 2016Review
- Insights into the structure and function of HV1 from a meta-analysis of mutation studies.The Journal of general physiology · 2016Review
- Acid-inducible proton influx currents in the plasma membrane of murine osteoclast-like cells.Pflugers Archiv : European journal of physiology · 2016Article
- Mechanism for attenuated outward conductance induced by mutations in the cytoplasmic pore of Kir2.1 channels.Scientific reports · 2015Article
- Tryptophan 207 is crucial to the unique properties of the human voltage-gated proton channel, hHV1.The Journal of general physiology · 2015Article
- Voltage-Gated Proton Channels as Novel Drug Targets: From NADPH Oxidase Regulation to Sperm Biology.Antioxidants & redox signaling · 2015Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Voltage-gated proton channels are found in many different types of cells, where they facilitate proton movement through the membrane. The mechanism of proton permeation through the channel is an issue of long-term interest, but it remains an open question. To address this issue, we examined the temperature dependence of proton permeation. Under whole cell recordings, rapid temperature changes within a few milliseconds were imposed. This method allowed for the measurement of current amplitudes immediately before and after a temperature jump, from which the ratios of these currents (Iratio) were determined. The use of Iratio for evaluating the temperature dependence minimized the contributions of factors other than permeation. Temperature jumps of various degrees (DeltaT, -15 to 15 degrees C) were applied over a wide temperature range (4-49 degrees C), and the Q10s for the proton currents were evaluated from the Iratios. Q10 exhibited a high temperature dependence, varying from 2.2 at 10 degrees C to 1.3 at 40 degrees C. This implies that processes with different temperature dependencies underlie the observed Q10. A novel resistivity pulse method revealed that the access resistance with its low temperature dependence predominated in high temperature ranges. The measured temperature dependence of Q10 was decomposed into Q10 of the channel and of the access resistances. Finally, the Q10 for proton permeation through the voltage-gated proton channel itself was calculated and found to vary from 2.8 at 5 degrees C to 2.2 at 45 degrees C, as expected for an activation enthalpy of 64 kJ/mol. The thermodynamic features for proton permeation through proton-selective channels were discussed for the underlying mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.