ArticleGenetics2009
Cross-species RNAi rescue platform in Drosophila melanogaster.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 46 citations in OpenAlex.
- Cross-species RNAi therapy via AAV delivery alleviates neuropathic pain by targeting GCH1.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2025Article
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- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
- The cecropin-prophenoloxidase regulatory mechanism is a cross-species physiological function in mosquitoes.iScience · 2022Article
- Article
- Generating and working with Drosophila cell cultures: Current challenges and opportunities.Wiley interdisciplinary reviews. Developmental biology · 2019Review
- Alcohol sedation in adultCommunications biology · 2019Article
- Review
- Article
- RNAi screening comes of age: improved techniques and complementary approaches.Nature reviews. Molecular cell biology · 2014Review
- RNAi screening in Drosophila cells and in vivo.Methods (San Diego, Calif.) · 2014Review
- Chemical mutagens, transposons, and transgenes to interrogate gene function in Drosophila melanogaster.Methods (San Diego, Calif.) · 2014Review
- Article
- Review
- Article
- MTERF3 regulates mitochondrial ribosome biogenesis in invertebrates and mammals.PLoS genetics · 2013Article
- Article
- Multispecies-compatible antitumor effects of a cross-species small-interfering RNA against mammalian target of rapamycin.Cellular and molecular life sciences : CMLS · 2012Article
- Kinesin heavy chain function in Drosophila glial cells controls neuronal activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- FlyRNAi.org--the database of the Drosophila RNAi screening center: 2012 update.Nucleic acids research · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
RNAi-mediated gene knockdown in Drosophila melanogaster is a powerful method to analyze loss-of-function phenotypes both in cell culture and in vivo. However, it has also become clear that false positives caused by off-target effects are prevalent, requiring careful validation of RNAi-induced phenotypes. The most rigorous proof that an RNAi-induced phenotype is due to loss of its intended target is to rescue the phenotype by a transgene impervious to RNAi. For large-scale validations in the mouse and Caenorhabditis elegans, this has been accomplished by using bacterial artificial chromosomes (BACs) of related species. However, in Drosophila, this approach is not feasible because transformation of large BACs is inefficient. We have therefore developed a general RNAi rescue approach for Drosophila that employs Cre/loxP-mediated recombination to rapidly retrofit existing fosmid clones into rescue constructs. Retrofitted fosmid clones carry a selection marker and a phiC31 attB site, which facilitates the production of transgenic animals. Here, we describe our approach and demonstrate proof-of-principle experiments showing that D. pseudoobscura fosmids can successfully rescue RNAi-induced phenotypes in D. melanogaster, both in cell culture and in vivo. Altogether, the tools and method that we have developed provide a gold standard for validation of Drosophila RNAi experiments.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.