ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2010
The cytoplasmic adaptor protein X11alpha and extracellular matrix protein Reelin regulate ApoE receptor 2 trafficking and cell movement.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Suppression of the amyloidogenic metabolism of APP and the accumulation of Aβ by alcadein α in the brain during aging.Scientific reports · 2024Article
- Interrogation and validation of the interactome of neuronal Munc18-interacting Mint proteins with AlphaFold2.The Journal of biological chemistry · 2024Article
- Reelin Signaling in Neurodevelopmental Disorders and Neurodegenerative Diseases.Brain sciences · 2023Review
- Human APOER2 Isoforms Have Differential Cleavage Events and Synaptic Properties.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Single molecule, long-read Apoer2 sequencing identifies conserved and species-specific splicing patterns.Genomics · 2022Article
- Reelin Functions, Mechanisms of Action and Signaling Pathways During Brain Development and Maturation.Biomolecules · 2020Review
- ApoER2: Functional Tuning Through Splicing.Frontiers in molecular neuroscience · 2020Review
- Article
- The Reelin Receptors Apolipoprotein E receptor 2 (ApoER2) and VLDL Receptor.International journal of molecular sciences · 2018Review
- GRIP1 Binds to ApoER2 and EphrinB2 to Induce Activity-Dependent AMPA Receptor Insertion at the Synapse.Cell reports · 2017Article
- Reelin: Neurodevelopmental Architect and Homeostatic Regulator of Excitatory Synapses.The Journal of biological chemistry · 2017Review
- Functional Roles of the Interaction of APP and Lipoprotein Receptors.Frontiers in molecular neuroscience · 2017Review
- Canonical and Non-canonical Reelin Signaling.Frontiers in cellular neuroscience · 2016Review
- Scaffold protein X11α interacts with kalirin-7 in dendrites and recruits it to Golgi outposts.The Journal of biological chemistry · 2014Article
- Differential splicing and glycosylation of Apoer2 alters synaptic plasticity and fear learning.Science signaling · 2014Article
- Altered neurotransmission in the lateral amygdala in aged human apoE4 targeted replacement mice.Neurobiology of aging · 2014Article
- The upside of APP at synapses.CNS neuroscience & therapeutics · 2012Review
- Fyn kinase regulates the association between amyloid precursor protein and Dab1 by promoting their localization to detergent-resistant membranes.Journal of neurochemistry · 2011Article
- Article
- Platelet adhesion and degranulation induce pro-survival and pro-angiogenic signalling in ovarian cancer cells.PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
The goal of this study was to determine the effect of X11alpha on ApoE receptor 2 (ApoEr2) trafficking and the functional significance of this interaction on cell movement in MCF 10A epithelial cells. We found that X11alpha increased surface levels of ApoEr2 by 64% compared to vector control, as determined by surface protein biotinylation. To examine the functional significance of this effect, we tested whether ApoEr2 played a novel role in cell movement in a wound-healing assay. We found that overexpression of ApoEr2 in MCF 10A cells increased cell migration velocity by 87% (P<0.01, n=4) compared to GFP control. Cotransfection of X11alpha had an additive effect on average velocity compared to ApoEr2 alone (13%; P<0.05, n=4). In addition, we tested whether ApoEr2 ligands altered the effect of ApoEr2 on cell movement. We found that treatment with concentrated medium containing the extracellular matrix protein Reelin, but not control medium, further increased the velocity of ApoEr2- but not APP-transfected cells (20%; P<0.001, n=4). Similarly, Reelin treatment increased cell velocity in the presence of ApoEr2 and X11alpha (10%; P<0.05, n=4). In the present study, we are the first to demonstrate that ApoEr2 regulates cell movement, and both X11alpha and Reelin enhance this effect.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.