ArticleExperimental cell research2009
Electrical stimulation of human embryonic stem cells: cardiac differentiation and the generation of reactive oxygen species.
Article in Experimental cell research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
98 citing papers in PubMed.
- Review
- A validated in vitro approach for low-frequency alternating-current stimulation of stem cells: From electrode characterization to biological responses.Protoplasma · 2026Article
- Development of low-cost electrical stimulation device to promote hiPSC-CM differentiation and functionality.APL bioengineering · 2026Article
- Modular bioreactor for multi-well electrical stimulation ofLab on a chip · 2026Article
- Parameter-resolved AC electrical stimulation links electrical system characteristics to early transcriptional responses in human stem cells.Frontiers in bioengineering and biotechnology · 2026Article
- Review
- Harnessing native blueprints for designing bioinks to bioprint functional cardiac tissue.iScience · 2025Review
- Article
- Analysis of the role of perfusion, mechanical, and electrical stimulation in bioreactors for cardiac tissue engineering.Bioprocess and biosystems engineering · 2024Review
- Article
- Electrical pulse stimulation parameters modulate N2a neuronal differentiation.Cell death discovery · 2024Article
- Computer-aided engineering and additive manufacturing for bioreactors in tissue engineering: State of the art and perspectives.Biophysics reviews · 2023Review
- Stem Cell Differentiation into Cardiomyocytes: Current Methods and Emerging Approaches.Stem cell reviews and reports · 2022Review
- Optical Control of Tissue Regeneration through Photostimulation of Organic Semiconducting Nanoparticles.Advanced healthcare materials · 2022Article
- Nanosecond pulsed electric fields prime mesenchymal stem cells to peptide ghrelin and enhance chondrogenesis and osteochondral defect repair in vivo.Science China. Life sciences · 2022Article
- Physiological Electric Field: A Potential Construction Regulator of Human Brain Organoids.International journal of molecular sciences · 2022Review
- Integration of clinical perspective into biomimetic bioreactor design for orthopedics.Journal of biomedical materials research. Part B, Applied biomaterials · 2022Review
- Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation.International journal of molecular sciences · 2022Article
- Electrical Stimulation and Cellular Behaviors in Electric Field in Biomedical Research.Materials (Basel, Switzerland) · 2021Review
- Redox Homeostasis and Regulation in Pluripotent Stem Cells: Uniqueness or Versatility?International journal of molecular sciences · 2021Review
38 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
Abstract
Exogenous electric fields have been implied in cardiac differentiation of mouse embryonic stem cells and the generation of reactive oxygen species (ROS). In this work, we explored the effects of electrical field stimulation on ROS generation and cardiogenesis in embryoid bodies (EBs) derived from human embryonic stem cells (hESC, line H13), using a custom-built electrical stimulation bioreactor. Electrical properties of the bioreactor system were characterized by electrochemical impedance spectroscopy (EIS) and analysis of electrical currents. The effects of the electrode material (stainless steel, titanium-nitride-coated titanium, titanium), length of stimulus (1 and 90 s) and age of EBs at the onset of electrical stimulation (4 and 8 days) were investigated with respect to ROS generation. The amplitude of the applied electrical field was 1 V/mm. The highest rate of ROS generation was observed for stainless steel electrodes, for signal duration of 90 s and for 4-day-old EBs. Notably, comparable ROS generation was achieved by incubation of EBs with 1 nM H(2)O(2). Cardiac differentiation in these EBs was evidenced by spontaneous contractions, expression of troponin T and its sarcomeric organization. These results imply that electrical stimulation plays a role in cardiac differentiation of hESCs, through mechanisms associated with the intracellular generation of ROS.
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Registered trials
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