ArticleBiophysical journal2009
Segregated phases in pulmonary surfactant membranes do not show coexistence of lipid populations with differentiated dynamic properties.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 97 citations in OpenAlex.
- Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled Lipids for Studying Lipid-Protein Interaction in Model and Biomembranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Modulation of Pulmonary Fibrosis by Pulmonary Surfactant-Associated Phosphatidylethanolamine In Vitro and In Vivo.International journal of molecular sciences · 2025Article
- Exposure to Aldehyde Cherry e-Liquid Flavoring and Its Vaping Byproduct Disrupt Pulmonary Surfactant Biophysical Function.Environmental science & technology · 2024Article
- Surfactant Proteins SP-B and SP-C in Pulmonary Surfactant Monolayers: Physical Properties Controlled by Specific Protein-Lipid Interactions.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- A recipe for a good clinical pulmonary surfactant.Biomedical journal · 2022Review
- Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- Pulmonary route of administration is instrumental in developing therapeutic interventions against respiratory diseases.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2020Review
- The Synergy of Membranotropic Effect of the Two PlaThe Journal of membrane biology · 2020Article
- Alveolar lipids in pulmonary disease. A review.Lipids in health and disease · 2020Review
- Understanding the Functional Properties of Lipid Heterogeneity in Pulmonary Surfactant Monolayers at the Atomistic Level.Frontiers in cell and developmental biology · 2020Article
- Addressing Differentiation in Live Human Keratinocytes by Assessment of Membrane Packing Order.Frontiers in cell and developmental biology · 2020Article
- Interfacial rheology and direct imaging reveal domain-templated network formation in phospholipid monolayers penetrated by fibrinogen.Soft matter · 2019Article
- Lipophilic Allergens, Different Modes of Allergen-Lipid Interaction and Their Impact on Asthma and Allergy.Frontiers in immunology · 2019Review
- The Perturbation of Pulmonary Surfactant by Bacterial Lipopolysaccharide and Its Reversal by Polymyxin B: Function and Structure.International journal of molecular sciences · 2018Article
- Evolution and mechanics of mixed phospholipid fibrinogen monolayers.Journal of the Royal Society, Interface · 2018Article
- Controlled hypothermia may improve surfactant function in asphyxiated neonates with or without meconium aspiration syndrome.PloS one · 2018Article
- Divide & Conquer: Surfactant Protein SP-C and Cholesterol Modulate Phase Segregation in Lung Surfactant.Biophysical journal · 2017Article
- Effect of Lung Surfactant Protein SP-C and SP-C-Promoted Membrane Fragmentation on Cholesterol Dynamics.Biophysical journal · 2016Article
- Spectral imaging to measure heterogeneity in membrane lipid packing.Chemphyschem : a European journal of chemical physics and physical chemistry · 2015Article
- Surfactant secretion in LRRK2 knock-out rats: changes in lamellar body morphology and rate of exocytosis.PloS one · 2014Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The composition of pulmonary surfactant membranes and films has evolved to support a complex lateral structure, including segregation of ordered/disordered phases maintained up to physiological temperatures. In this study, we have analyzed the temperature-dependent dynamic properties of native surfactant membranes and membranes reconstituted from two surfactant hydrophobic fractions (i.e., all the lipids plus the hydrophobic proteins SP-B and SP-C, or only the total lipid fraction). These preparations show micrometer-sized fluid ordered/disordered phase coexistence, associated with a broad endothermic transition ending close to 37 degrees C. However, both types of membrane exhibit uniform lipid mobility when analyzed by electron paramagnetic resonance with different spin-labeled phospholipids. A similar feature is observed with pulse-field gradient NMR experiments on oriented membranes reconstituted from the two types of surfactant hydrophobic extract. These latter results suggest that lipid dynamics are similar in the coexisting fluid phases observed by fluorescence microscopy. Additionally, it is found that surfactant proteins significantly reduce the average intramolecular lipid mobility and translational diffusion of phospholipids in the membranes, and that removal of cholesterol has a profound impact on both the lateral structure and dynamics of surfactant lipid membranes. We believe that the particular lipid composition of surfactant imposes a highly dynamic framework on the membrane structure, as well as maintains a lateral organization that is poised at the edge of critical transitions occurring under physiological conditions.
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