ArticleBiophysical journal2009
Contact-mode high-resolution high-speed atomic force microscopy movies of the purple membrane.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 60 citations in OpenAlex.
- Seeing the unseen: High-resolution AFM imaging captures antibiotic action in bacterial membranes.Nature communications · 2022Article
- Fast and high-resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM.Nature protocols · 2018Article
- [Progress in the applications of high-speed atomic force microscopy in cell biology].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2018Review
- Optical and force nanoscopy in microbiology.Nature microbiology · 2016Review
- Bone quality assessment techniques: geometric, compositional, and mechanical characterization from macroscale to nanoscale.Clinical reviews in bone and mineral metabolism · 2016Article
- Fast, multi-frequency, and quantitative nanomechanical mapping of live cells using the atomic force microscope.Scientific reports · 2015Article
- Low pH modulates the macroorganization and thermal stability of PSII supercomplexes in grana membranes.Biophysical journal · 2015Article
- Filming biomolecular processes by high-speed atomic force microscopy.Chemical reviews · 2014Review
- Lateral organization of biological membranes: role of long-range interactions.European biophysics journal : EBJ · 2013Article
- Large-scale analysis of high-speed atomic force microscopy data sets using adaptive image processing.Beilstein journal of nanotechnology · 2012Article
- Experimental evidence for membrane-mediated protein-protein interaction.Biophysical journal · 2010Article
- Sampling protein form and function with the atomic force microscope.Molecular & cellular proteomics : MCP · 2010Review
- Heterogeneous diffusion of a membrane-bound pHLIP peptide.Biophysical journal · 2010Article
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-speed atomic force microscopy (HS-AFM) is becoming a reference tool for the study of dynamic biological processes. The spatial and time resolutions of HS-AFM are on the order of nanometers and milliseconds, respectively, and allow structural and functional characterization of biological processes at the single-molecule level. In this work we present contact-mode HS-AFM movies of purple membranes containing two-dimensional arrays of bacteriorhodopsin (bR). In high-resolution movies acquired at a 100 ms frame acquisition time, the substructure on individual bR trimers was visualized. In regions in between different bR arrays, dynamic topographies were observed and interpreted as motion of the bR trimers. Similarly, motion of bR monomers in the vicinity of lattice defects in the purple membrane was observed. Our findings indicate that the bR arrays are in a mobile association-dissociation equilibrium. HS-AFM on membranes provides novel perspectives for analyzing the membrane diffusion processes of nonlabeled molecules.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.