ArticleBiophysical journal2009
Effect of energy metabolism on protein motility in the bacterial outer membrane.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- Protection of bacteriophage-sensitiveProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Complex Diffusion in Bacteria.Advances in experimental medicine and biology · 2020Review
- Measuring the Conformational Distance of GPCR-related Proteins Using a Joint-based Descriptor.Scientific reports · 2017Article
- Illumination of growth, division and secretion by metabolic labeling of the bacterial cell surface.FEMS microbiology reviews · 2015Review
- Transcriptional pathways associated with the slow growth phenotype of transformed Anaplasma marginale.BMC genomics · 2013Article
- Absence of long-range diffusion of OmpA in E. coli is not caused by its peptidoglycan binding domain.BMC microbiology · 2013Article
- Force probing of individual molecules inside the living cell is now a reality.Nature chemical biology · 2012Review
- Nonthermal ATP-dependent fluctuations contribute to the in vivo motion of chromosomal loci.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Analysis of surface protein expression reveals the growth pattern of the gram-negative outer membrane.PLoS computational biology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate the energy dependence of the motion of a porin, the lambda-receptor, in the outer membrane of living Escherichia coli by single molecule investigations. By poisoning the bacteria with arsenate and azide, the bacterial energy metabolism was stopped. The motility of individual lambda-receptors significantly and rapidly decreased upon energy depletion. We suggest two different causes for the ceased motility upon comprised energy metabolism: One possible cause is that the cell uses energy to actively wiggle its proteins, this energy being one order-of-magnitude larger than thermal energy. Another possible cause is an induced change in the connection between the lambda-receptor and the membrane structure, for instance by a stiffening of part of the membrane structure. Treatment of the cells with ampicillin, which directly targets the bacterial cell wall by inhibiting cross-linking of the peptidoglycan layer, had an effect similar to energy depletion and the motility of the lambda-receptor significantly decreased. Since the lambda-receptor is closely linked to the peptidoglycan layer, we propose that lambda-receptor motility is directly coupled to the constant and dynamic energy-consuming reconstruction of the peptidoglycan layer. The result of this motion could be to facilitate transport of maltose-dextrins through the porin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.