Evidence map›Paper›PMID 19718033›Full record

ArticleNature methods2009

Nanoscale 3D cellular imaging by axial scanning transmission electron tomography.

Martin F Hohmann-Marriott, Alioscka A Sousa, Afrouz A Azari, Svetlana Glushakova, Guofeng Zhang, Joshua Zimmerberg, Richard D Leapman

Abstract read
In one paragraph

Article in Nature methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.

0numbers the graph read from it
0cells of the map it votes in
66citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

66 citing papers in PubMed.

  1. Review
  2. High-Pressure Freezing EM Tomography of Entire Ribbon Synapses in the Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025
    Article
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  4. Article
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  8. Review
  9. Article
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  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article

6 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Martin F Hohmann-MarriottNational Institute of Biomedical Imaging and Bioengineering, Bethesda, Maryland, USA.
Alioscka A Sousa
Afrouz A Azari
Svetlana Glushakova
Guofeng Zhang
Joshua Zimmerberg
Richard D Leapman

Funding

Membrane Remodeling in Viral Infection, Parasite Replication, and Traumatic Brain InjuryZIAHD001409 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI ZIMMERBERG, JOSHUA · 2009 to 2025
$29.9M
Scanning Transmission Electron Tomography of Biological StructuresZIAEB000046 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI LEAPMAN, RICHARD · 2009 to 2025
$13.0M
Scanning Transmission Electron Tomography of Biological StructuresZ01EB000046 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI LEAPMAN, RICHARD · 2007 to 2008
$95k
Intramural NIH HHS Z01 EB000046
6 · The paper itself

Abstract

Electron tomography provides three-dimensional structural information about supramolecular assemblies and organelles in a cellular context, but image degradation, caused by scattering of transmitted electrons, limits applicability in specimens thicker than 300 nm. We found that scanning transmission electron tomography of 1,000-nm-thick samples using axial detection provided resolution comparable to that of conventional electron tomography. We demonstrated the method by reconstructing a human erythrocyte infected with the malaria parasite Plasmodium falciparum.

Indexed as

AnimalsCells, CulturedElectron Microscope TomographyErythrocytesHumansImage EnhancementImaging, Three-DimensionalNanotechnologyPlasmodium falciparum

Identifiers

PMID19718033
PMCPMC2755602

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.