ArticleThe American journal of pathology2009
Repopulation efficiencies of adult hepatocytes, fetal liver progenitor cells, and embryonic stem cell-derived hepatic cells in albumin-promoter-enhancer urokinase-type plasminogen activator mice.
Article in The American journal of pathology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 106 citations in OpenAlex.
- Production of factor VIII by human liver sinusoidal endothelial cells transplanted in immunodeficient uPA mice.PloS one · 2013Trial
- Transient culture of adult human hepatocytes as organoids improves engineered liver engraftment.Biomaterials · 2027Article
- Cell therapy in end-stage liver disease: replace and remodel.Stem cell research & therapy · 2023Review
- Therapeutic Cell Repopulation of the Liver: From Fetal Rat Cells to Synthetic Human Tissues.Cells · 2023Review
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- Preparation of Functional Human Hepatocytes Ex Vivo.Methods in molecular biology (Clifton, N.J.) · 2022Article
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- A critical look: Challenges in differentiating human pluripotent stem cells into desired cell types and organoids.Wiley interdisciplinary reviews. Developmental biology · 2020Review
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- Glypican-3-Specific Antibody Drug Conjugates Targeting Hepatocellular Carcinoma.Hepatology (Baltimore, Md.) · 2019Article
- Biologically inspired approaches to enhance human organoid complexity.Development (Cambridge, England) · 2019Review
- Bioengineered functional humanized livers: An emerging supportive modality to bridge the gap of organ transplantation for management of end-stage liver diseases.World journal of hepatology · 2018Review
- A Roadmap for Human Liver Differentiation from Pluripotent Stem Cells.Cell reports · 2018Article
- Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells.Journal of visualized experiments : JoVE · 2016Article
- Humanized mice efficiently engrafted with fetal hepatoblasts and syngeneic immune cells develop human monocytes and NK cells.Journal of hepatology · 2016Article
- In vivo models of hepatitis B and C virus infection.FEBS letters · 2016Review
- Stem Cell Therapies for Treatment of Liver Disease.Biomedicines · 2016Review
- Transplantation of hESC-derived hepatocytes protects mice from liver injury.Stem cell research & therapy · 2015Article
- Determinants of hepatitis B and delta virus host tropism.Current opinion in virology · 2015Review
Corrections and comments
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Authors and funding
19 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fetal liver progenitor cell suspensions (FLPC) and hepatic precursor cells derived from embryonic stem cells (ES-HPC) represent a potential source for liver cell therapy. However, the relative capacity of these cell types to engraft and repopulate a recipient liver compared with adult hepatocytes (HC) has not been comprehensively assessed. We transplanted mouse and human HC, FLPC, and ES-HPC into a new immunodeficient mouse strain (Alb-uPA(tg(+/-))Rag2(-/-)gamma(c)(-/-) mice) and estimated the percentages of HC after 3 months. Adult mouse HC repopulated approximately half of the liver mass (46.6 +/- 8.0%, 1 x 10(6) transplanted cells), whereas mouse FLPC derived from day 13.5 and 11.5 post conception embryos generated only 12.1 +/- 3.0% and 5.1 +/- 1.1%, respectively, of the recipient liver and smaller cell clusters. Adult human HC and FLPC generated overall less liver tissue than mouse cells and repopulated 10.0 +/- 3.9% and 2.7 +/- 1.1% of the recipient livers, respectively. Mouse and human ES-HPC did not generate HC clusters in our animal model. We conclude that, in contrast to expectations, adult HC of human and mouse origin generate liver tissue more efficiently than cells derived from fetal tissue or embryonic stem cells in a highly immunodeficient Alb-uPA transgenic mouse model system. These results have important implications in the context of selecting the optimal strategy for human liver cell therapies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.