ArticleJournal of bacteriology2009
Mechanism of transcriptional activation by Pseudomonas aeruginosa ExsA.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed.
- Coordination of quorum sensing with T3SS and T6SS: Bacterial strategies for combating fungal pathogens.Virulence · 2026Review
- TypA Interacts with ExsA to Suppress Type III Secretion System inMicroorganisms · 2026Article
- Simultaneous inhibition of bacterial virulence and anti-phage defense systems by synergistic bacteriophage counter-defense proteins.The EMBO journal · 2026Article
- GlnK Regulates the Type III Secretion System by Modulating NtrB-NtrC Homeostasis inMicroorganisms · 2026Article
- Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis.Communications biology · 2025Article
- Uncovering the Multifaceted Role ofMicroorganisms · 2025Article
- TargIDe: a machine-learning workflow for target identification of molecules with antibiofilm activity against Pseudomonas aeruginosa.Journal of computer-aided molecular design · 2023Article
- MvaT binds to the PFrontiers in cellular and infection microbiology · 2023Article
- Genomic and Metabolic Characteristics of the Pathogenicity inInternational journal of molecular sciences · 2021Review
- Article
- Backbone Interactions Between Transcriptional Activator ExsA and Anti-Activator ExsD Facilitate Regulation of the Type III Secretion System in Pseudomonas aeruginosa.Scientific reports · 2020Article
- HilD, HilC, and RtsA Form Homodimers and Heterodimers To Regulate Expression of theJournal of bacteriology · 2020Article
- Article
- Pseudomonas aeruginosa ExsA Regulates a Metalloprotease, ImpA, That Inhibits Phagocytosis of Macrophages.Infection and immunity · 2019Article
- RNase E Promotes Expression of Type III Secretion System Genes in Pseudomonas aeruginosa.Journal of bacteriology · 2019Article
- Transcriptional regulation of fatty acid cis-trans isomerization in the solvent-tolerant soil bacterium, Pseudomonas putida F1.Environmental microbiology · 2019Article
- The Transcriptional Regulator HlyU Positively Regulates Expression ofJournal of bacteriology · 2018Article
- Post-transcriptional regulation of type III secretion in plant and animal pathogens.Current opinion in microbiology · 2017Review
- Fis Regulates Type III Secretion System by Influencing the Transcription ofFrontiers in microbiology · 2017Article
- Design and characterization of a polyamine derivative inhibiting the expression of type III secretion system in Pseudomonas aeruginosa.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
ExsA is a transcriptional activator of the Pseudomonas aeruginosa type III secretion system (T3SS). The T3SS consists of >40 genes organized within 10 transcriptional units, each of which is controlled by the transcriptional activator ExsA. ExsA-dependent promoters contain two adjacent ExsA binding sites that when occupied protect the -30 to -70 region from DNase I cleavage. The promoters also possess regions bearing strong resemblance to the consensus -10 and -35 regions of sigma(70)-dependent promoters. The spacing distance between the putative -10 and -35 regions of ExsA-dependent promoters, however, is increased by 4 to 5 bp compared to that in typical sigma(70)-dependent promoters. In the present study, we demonstrate that ExsA-dependent transcriptional activation requires a 21- or 22-bp spacer length between the -10 and -35 regions. Despite the atypical spacing in this region, in vitro transcription assays using sigma(70)-saturated RNA polymerase holoenzyme (RNAP-sigma(70)) confirm that ExsA-dependent promoters are indeed sigma(70) dependent. Potassium permanganate footprinting experiments indicate that ExsA facilitates an early step in transcriptional initiation. Although RNAP-sigma(70) binds to the promoters with low affinity in the absence of ExsA, the activator stimulates transcription by enhancing recruitment of RNAP-sigma(70) to the P(exsC) and P(exsD) promoters. Abortive initiation assays confirm that ExsA enhances the equilibrium binding constant for RNAP while having only a modest effect on the isomerization rate constant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.