ArticleJournal of bacteriology2009
Two essential arginine residues in the T components of energy-coupling factor transporters.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- The S-component fold: a link between bacterial transporters and receptors.Communications biology · 2024Article
- Expulsion mechanism of the substrate-translocating subunit in ECF transporters.Nature communications · 2023Article
- ATP-Binding Cassette Transporters: Snap-on Complexes?Sub-cellular biochemistry · 2022Article
- Article
- A Novel Prokaryote-Type ECF/ABC Transporter Module in Chloroplast Metal Homeostasis.Frontiers in plant science · 2019Article
- Structure and mechanism of a group-I cobalt energy coupling factor transporter.Cell research · 2017Article
- ATP-dependent Conformational Changes Trigger Substrate Capture and Release by an ECF-type Biotin Transporter.The Journal of biological chemistry · 2015Article
- ATP binding drives substrate capture in an ECF transporter by a release-and-catch mechanism.Nature structural & molecular biology · 2015Article
- Structure of a pantothenate transporter and implications for ECF module sharing and energy coupling of group II ECF transporters.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Planar substrate-binding site dictates the specificity of ECF-type nickel/cobalt transporters.Cell research · 2014Article
- Structural and mechanistic insights into prokaryotic energy-coupling factor transporters.Nature reviews. Microbiology · 2014Article
- Unveiling the gating mechanism of ECF transporter RibU.Scientific reports · 2013Article
- Structural basis for a homodimeric ATPase subunit of an ECF transporter.Protein & cell · 2013Article
- Solitary BioY proteins mediate biotin transport into recombinant Escherichia coli.Journal of bacteriology · 2013Article
- Article
- Crystal structure of a folate energy-coupling factor transporter from Lactobacillus brevis.Nature · 2013Article
- Assembly and mechanism of a group II ECF transporter.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Essential amino acid residues of BioY reveal that dimers are the functional S unit of the Rhodobacter capsulatus biotin transporter.Journal of bacteriology · 2012Article
- Structural divergence of paralogous S components from ECF-type ABC transporters.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- RibM from Streptomyces davawensis is a riboflavin/roseoflavin transporter and may be useful for the optimization of riboflavin production strains.BMC biotechnology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Energy-coupling factor (ECF) transporters, a recently discovered class of importers of micronutrients, are composed of a substrate-specific transmembrane component (S component) and a conserved energy-coupling module consisting of a transmembrane protein (T component) and pairs of ABC ATPases (A proteins). Based on utilization of a dedicated (subclass I) or shared (subclass II) energy-coupling module, ECF systems fall into two subclasses. The T components are the least-characterized proteins of ECF importers, and their function is essentially unknown. Using RcBioN and LmEcfT, the T units of the subclass I biotin transporter (RcBioMNY) of a gram-negative bacterium and of the subclass II folate, pantothenate, and riboflavin transporters of a lactic acid bacterium, respectively, we analyzed the role of two strongly conserved short motifs, each containing an arginine residue. Individual replacement of the two Arg residues in RcBioN reduced ATPase activity, an indicator of the transporter function, by two-thirds without affecting the modular assembly of the RcBioMNY complex. A double Arg-to-Glu replacement destroyed the complex and abolished ATPase activity. The corresponding single mutation in motif II of LmEcfT, as well as a double mutation, led to loss of the T unit from the subclass II ECF transporters and inactivated these systems. A single Arg-to-Glu replacement in motif I, however, abolished vitamin uptake activity without affecting assembly of the modules. Our results indicate that the conserved motif I in T components is essential for intramolecular signaling and, in cooperation with motif II, for subunit assembly of modular ECF transporters.
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