ArticleJournal of bacteriology2009
Single-residue changes in the C-terminal disulfide-bonded loop of the Pseudomonas aeruginosa type IV pilin influence pilus assembly and twitching motility.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Development of a sensor for disulfide bond formation in diverse bacteria.Journal of bacteriology · 2024Article
- Understanding a Core Pilin of the Type IVa Pili ofJournal of microbiology and biotechnology · 2024Article
- Discovery and characterization of a new class of O-linking oligosaccharyltransferases from the Moraxellaceae family.Glycobiology · 2023Article
- Phage Resistance Evolution Induces the Sensitivity of Specific Antibiotics in Pseudomonas aeruginosa PAO1.Microbiology spectrum · 2022Article
- Protein Nanotubes: From Bionanotech towards Medical Applications.Biomedicines · 2019Review
- Inhibition of Pseudomonas aeruginosa and Mycobacterium tuberculosis disulfide bond forming enzymes.Molecular microbiology · 2019Article
- Phage Morons Play an Important Role in Pseudomonas aeruginosa Phenotypes.Journal of bacteriology · 2018Article
- Diversity and Evolution of Myxobacterial Type IV Pilus Systems.Frontiers in microbiology · 2018Article
- Pseudomonas aeruginosa defends against phages through type IV pilus glycosylation.Nature microbiology · 2018Article
- A Pilin Region Affecting Host Range of theFrontiers in microbiology · 2018Article
- Fingerprint Analysis and Identification of Strains ST309 as a Potential High Risk Clone in aFrontiers in microbiology · 2017Article
- Modeling and Simulating the Dynamics of Type IV Pili Extension of Pseudomonas aeruginosa.Biophysical journal · 2016Article
- Type IV Pilus Alignment Subcomplex Proteins PilN and PilO Form Homo- and Heterodimers in Vivo.The Journal of biological chemistry · 2016Article
- Temperate phages both mediate and drive adaptive evolution in pathogen biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Type IV pilins regulate their own expression via direct intramembrane interactions with the sensor kinase PilS.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Structural and functional studies of the Pseudomonas aeruginosa minor pilin, PilE.The Journal of biological chemistry · 2015Article
- Nanoscale Pulling of Type IV Pili Reveals Their Flexibility and Adhesion to Surfaces over Extended Lengths of the Pili.Biophysical journal · 2015Article
- Compounds targeting disulfide bond forming enzyme DsbB of Gram-negative bacteria.Nature chemical biology · 2015Article
- High-resolution structure of a type IV pilin from the metal-reducing bacterium Shewanella oneidensis.BMC structural biology · 2015Article
- The platform protein is essential for type IV pilus biogenesis.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PilA, the major pilin subunit of Pseudomonas aeruginosa type IV pili (T4P), is a principal structural component. PilA has a conserved C-terminal disulfide-bonded loop (DSL) that has been implicated as the pilus adhesinotope. Structural studies have suggested that DSL is involved in intersubunit interactions within the pilus fiber. PilA mutants with single-residue substitutions, insertions, or deletions in the DSL were tested for pilin stability, pilus assembly, and T4P function. Mutation of either Cys residue of the DSL resulted in pilins that were unable to assemble into fibers. Ala replacements of the intervening residues had a range of effects on assembly or function, as measured by changes in surface pilus expression and twitching motility. Modification of the C-terminal P-X-X-C type II beta-turn motif, which is one of the few highly conserved features in pilins across various species, caused profound defects in assembly and twitching motility. Expression of pilins with suspected assembly defects in a pilA pilT double mutant unable to retract T4P allowed us to verify which subunits were physically unable to assemble. Use of two different PilA antibodies showed that the DSL may be an immunodominant epitope in intact pili compared with pilin monomers. Sequence diversity of the type IVa pilins likely reflects an evolutionary compromise between retention of function and antigenic variation. The consequences of DSL sequence changes should be evaluated in the intact protein since it is technically feasible to generate DSL-mimetic peptides with mutations that will not appear in the natural repertoire due to their deleterious effects on assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.