ArticleThe Journal of biological chemistry2009
Transforming growth factors beta coordinate cartilage and tendon differentiation in the developing limb mesenchyme.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 174 citations in OpenAlex.
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- Donor-dependent regulation of type II and X collagen deposition by early modulation of miR-335-5p and miR-1246 during chondrogenic commitment.Stem cell research & therapy · 2025Article
- Maize stover burning exposure accountable for remarkable environmental and health risk in broiler chickens.BMC veterinary research · 2025Article
- Proteoglycan Dysfunction: A Common Link Between Intervertebral Disc Degeneration and Skeletal Dysplasia.Neurospine · 2024Article
- Rebalancing SMAD7/SMAD3 Signaling Reduces Adhesion Formation during Flexor Tendon Healing.Journal of microbiology and biotechnology · 2023Article
- Article
- Wnt antagonism without TGFβ induces rapid MSC chondrogenesis via increasing AJ interactions and restricting lineage commitment.iScience · 2023Article
- The Functions and Mechanisms of Tendon Stem/Progenitor Cells in Tendon Healing.Stem cells international · 2023Review
- The TFEB-TGIF1 axis regulates EMT in mouse epicardial cells.Nature communications · 2022Article
- TGFβ1-transfected tendon stem cells promote tendon fibrosis.Journal of orthopaedic surgery and research · 2022Article
- Evaluation of CRISPR/Cas9 mediated TGIF gene editing to inhibit corneal fibrosis in vitro.Experimental eye research · 2022Article
- Comparison of Tendon Development Versus Tendon Healing and Regeneration.Frontiers in cell and developmental biology · 2022Review
- Tenogenic Induction From Induced Pluripotent Stem Cells Unveils the Trajectory Towards Tenocyte Differentiation.Frontiers in cell and developmental biology · 2022Article
- The homeobox gene TGIF1 is required for chicken ovarian cortical development and generation of the juxtacortical medulla.Development (Cambridge, England) · 2021Article
- Functional regeneration and repair of tendons using biomimetic scaffolds loaded with recombinant periostin.Nature communications · 2021Article
- Akt signaling is activated by TGFβ2 and impacts tenogenic induction of mesenchymal stem cells.Stem cell research & therapy · 2021Article
- Tendinopathy.Nature reviews. Disease primers · 2021Review
- Effects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors.Journal of inflammation research · 2021Article
- Tendon Tissue Repair in Prospective of Drug Delivery, Regenerative Medicines, and Innovative Bioscaffolds.Stem cells international · 2021Review
21 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transforming growth factor beta (TGFbeta) signaling has an increasing interest in regenerative medicine as a potential tool to repair cartilages, however the chondrogenic effect of this pathway in developing systems is controversial. Here we have analyzed the function of TGFbeta signaling in the differentiation of the developing limb mesoderm in vivo and in high density micromass cultures. In these systems highest signaling activity corresponded with cells at stages preceding overt chondrocyte differentiation. Interestingly treatments with TGFbetas shifted the differentiation outcome of the cultures from chondrogenesis to fibrogenesis. This phenotypic reprogramming involved down-regulation of Sox9 and Aggrecan and up-regulation of Scleraxis, and Tenomodulin through the Smad pathway. We further show that TGFbeta signaling up-regulates Sox9 in the in vivo experimental model system in which TGFbeta treatments induce ectopic chondrogenesis. Looking for clues explaining the dual role of TGFbeta signaling, we found that TGFbetas appear to be direct inducers of the chondrogenic gene Sox9, but the existence of transcriptional repressors of TGFbeta signaling modulates this role. We identified TGF-interacting factor Tgif1 and SKI-like oncogene SnoN as potential candidates for this inhibitory function. Tgif1 gene regulation by TGFbeta signaling correlated with the differential chondrogenic and fibrogenic effects of this pathway, and its expression pattern in the limb marks the developing tendons. In functional experiments we found that Tgif1 reproduces the profibrogenic effect of TGFbeta treatments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.