ArticleExperimental cell research2010
DNA methyltransferase inhibition induces mouse embryonic stem cell differentiation into endothelial cells.
Article in Experimental cell research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Modeling DNA methyltransferase function to predict epigenetic correlation patterns in healthy and cancer cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Vasculogenic skin reprogramming requires TET-mediated gene demethylation in fibroblasts for rescuing impaired perfusion in diabetes.Nature communications · 2024Article
- Knockdown of TPI in human dermal microvascular endothelial cells and its impact on angiogenesis in vitro.PloS one · 2023Article
- Combination of mesenchymal stem cells and three-dimensional collagen scaffold preserves ventricular remodeling in rat myocardial infarction model.World journal of stem cells · 2022Article
- Expression of vimentin, TPI and MAT2A in human dermal microvascular endothelial cells during angiogenesis in vitro.PloS one · 2022Article
- Potential epigenetic molecular regulatory networks in ocular neovascularization.Frontiers in genetics · 2022Review
- Epigenetic regulation of dental pulp stem cells and its potential in regenerative endodontics.World journal of stem cells · 2021Review
- Epigenetic Regulation of Endothelial Cell Function by Nucleic Acid Methylation in Cardiac Homeostasis and Disease.Cardiovascular drugs and therapy · 2021Review
- Microphysiological Modeling of the Human Endometrium.Tissue engineering. Part A · 2020Article
- Small molecule 2'-deoxycytidine differentiates human umbilical cord-derived MSCs into cardiac progenitors in vitro and their in vivo xeno-transplantation improves cardiac function.Molecular and cellular biochemistry · 2020Article
- Generation of Progesterone-Responsive Endometrial Stromal Fibroblasts from Human Induced Pluripotent Stem Cells: Role of the WNT/CTNNB1 Pathway.Stem cell reports · 2018Article
- The Mammalian Target of Rapamycin and DNA methyltransferase 1 axis mediates vascular endothelial dysfunction in response to disturbed flow.Scientific reports · 2017Article
- Integrative analysis of somatic mutations and transcriptomic data to functionally stratify breast cancer patients.BMC genomics · 2016Article
- Cancer Stem Cells: Dynamic Entities in an Ever-Evolving Paradigm.Biology and medicine (Aligarh) · 2015Article
- Mesenchymal stem cells: roles and relationships in vascularization.Tissue engineering. Part B, Reviews · 2014Review
- Endothelial heterogeneity in the umbilico-placental unit: DNA methylation as an innuendo of epigenetic diversity.Frontiers in pharmacology · 2014Review
- Epigenetic programming and risk: the birthplace of cardiovascular disease?Stem cell reviews and reports · 2013Review
- Identification of retinol binding protein 1 promoter hypermethylation in isocitrate dehydrogenase 1 and 2 mutant gliomas.Journal of the National Cancer Institute · 2012Article
- Epigenetic landscape of pluripotent stem cells.Antioxidants & redox signaling · 2012Review
- Neural stem cells exposed to BrdU lose their global DNA methylation and undergo astrocytic differentiation.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Understanding endothelial cell (EC) differentiation is a step forward in tissue engineering, controlling angiogenesis, and endothelial dysfunction. We hypothesized that epigenetic activation of EC lineage specification genes is an important mediator of embryonic stem cell (ESC) differentiation into EC. Mouse ESC was differentiated by removing leukemia inhibitory factor (LIF) from the maintenance media in the presence or absence of the specific DNA methyltransferase (DNMT) inhibitor 5'-aza-2'-deoxycytidine (aza-dC). Expression of EC specification and marker genes was monitored by quantitative PCR, western, immunocytochemistry, and flow cytometry. Functionality of differentiated EC was assessed by angiogenesis assay. The methylation status in the proximal promoter CpGs of the mediators of EC differentiation VEGF-A, BMP4, and EPAS-1 as well as of the mature EC marker VE-cadherin was determined by bisulfite sequencing. ESC differentiation resulted in repression of OCT4 expression in both the absence and presence of aza-dC treatment. However, significant increase in angiogenesis and expression of the mediators of EC differentiation and EC-specific genes was only observed in aza-dC-treated cells. The DNMT inhibition-mediated increase in EC specification and marker gene expression was not associated with demethylation of these genes. These studies suggest that DNMT inhibition is an efficient inducer of EC differentiation from ESC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.