ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2009
FISH and DAPI staining of the synaptonemal complex of the Nile tilapia (Oreochromis niloticus) allow orientation of the unpaired region of bivalent 1 observed during early pachytene.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 26 citations in OpenAlex.
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- Origin of a Giant Sex Chromosome.Molecular biology and evolution · 2021Article
- Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes.Journal of visualized experiments : JoVE · 2020Article
- Review
- Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum.Journal of genetics · 2016Article
- Surface-spreading technique of meiotic cells and immunodetection of synaptonemal complex proteins in teleostean fishes.Molecular cytogenetics · 2015Article
- Construction of a microsatellites-based linkage map for the white grouper (Epinephelus aeneus).G3 (Bethesda, Md.) · 2014Article
- Characterization of gonadal transcriptomes from Nile tilapia (Oreochromis niloticus) reveals differentially expressed genes.PloS one · 2013Article
- Integrating cytogenetics and genomics in comparative evolutionary studies of cichlid fish.BMC genomics · 2012Article
- Genetic and physical mapping of sex-linked AFLP markers in Nile tilapia (Oreochromis niloticus).Marine biotechnology (New York, N.Y.) · 2011Article
- Characterisation of the chromosome fusions in Oreochromis karongae.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Chromosome evolution in African cichlid fish: contributions from the physical mapping of repeated DNAs.Cytogenetic and genome research · 2010Article
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4 authors at 3 institutions in 2 countries.
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Abstract
Bivalent 1 of the synaptonemal complex (SC) in XY male Oreochromis niloticus shows an unpaired terminal region in early pachytene. This appears to be related to recombination suppression around a sex determination locus. To allow more detailed analysis of this, and unpaired regions in the karyotype of other Oreochromis species, we developed techniques for FISH on SC preparations, combined with DAPI staining. DAPI staining identified presumptive centromeres in SC bivalents, which appeared to correspond to the positions observed in the mitotic karyotype (the kinetochores could be identified only sporadically in silver-stained EM SC images). Furthermore, two BAC clones containing Dmo (dmrt4) and OniY227 markers that hybridize to known positions in chromosome pair 1 in mitotic spreads (near the centromere, Flpter 0.25, and the putative sex-determination locus, Flpter 0.57, respectively) were used as FISH probes on SCs to verify that the presumptive centromere identified by DAPI staining was located in the expected position. Visualization of both the centromere and FISH signals on bivalent 1 allowed the unpaired region to be positioned at Flpter 0.80 to 1.00, demonstrating that the unpaired region is located in the distal part of the long arm(s). Finally, differences between mitotic and meiotic measurements are discussed.
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