ArticlePlanta2009
Pleiotropic changes in Arabidopsis f5h and sct mutants revealed by large-scale gene expression and metabolite analysis.
Article in Planta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Sinapoylglucose: malate sinapoyltransferase SNG1 negatively regulates drought resistance by crosstalk with abscisic acid signaling in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- From Defense Executor to Engineering Target: Harnessing Lignin for Crop Resistance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Reducing the Sinapine Levels of Camelina sativa Seeds Through Targeted Genome Editing of REF1.Plant biotechnology journal · 2026Article
- Integrated transcriptomic and metabolomic analyses uncover the key pathways of Limonium bicolor in response to salt stress.Plant biotechnology journal · 2025Article
- Integrated physiological and transcriptomic analysis reveals the key pathways ofFrontiers in plant science · 2025Article
- Studying temperature's impact on Brassica napus resistance to identify key regulatory mechanisms using comparative metabolomics.Scientific reports · 2024Article
- Engineering a feedback inhibition-insensitive plant dihydrodipicolinate synthase to increase lysine content in Camelina sativa seeds.Transgenic research · 2022Article
- Phenylpropanoid Pathway Engineering: An Emerging Approach towards Plant Defense.Pathogens (Basel, Switzerland) · 2020Review
- SCARECROW-LIKE15 interacts with HISTONE DEACETYLASE19 and is essential for repressing the seed maturation programme.Nature communications · 2015Article
- Elucidating the genetic basis of antioxidant status in lettuce (Lactuca sativa).Horticulture research · 2015Article
- Brassica villosa, a system for studying non-glandular trichomes and genes in the Brassicas.Plant molecular biology · 2014Article
- Genetic enhancement of Brassica napus seed quality.Transgenic research · 2014Review
- The role of the secondary cell wall in plant resistance to pathogens.Frontiers in plant science · 2014Review
- A bacterial quercetin oxidoreductase QuoA-mediated perturbation in the phenylpropanoid metabolic network increases lignification with a concomitant decrease in phenolamides in Arabidopsis.Journal of experimental botany · 2013Article
- Modifying lignin to improve bioenergy feedstocks: strengthening the barrier against pathogens?Frontiers in plant science · 2013Article
- Molecular characterization of ferulate 5-hydroxylase gene from kenaf (Hibiscus cannabinus L.).TheScientificWorldJournal · 2013Article
- A rapid, simple method for the genetic discrimination of intact Arabidopsis thaliana mutant seeds using metabolic profiling by direct analysis in real-time mass spectrometry.Plant methods · 2011Article
- Overexpression of sinapine esterase BnSCE3 in oilseed rape seeds triggers global changes in seed metabolism.Plant physiology · 2011Article
- Cytochromes p450.The arabidopsis book · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrocinnamic acid esters, lignin, flavonoids, glucosinolates, and salicylic acid protect plants against UV exposure, oxidative stress, diseases, and herbivores. Through the phenylpropanoid pathway, certain Brassicaceae family members, including Arabidopsis thaliana and Brassica napus, accumulate large amounts of the anti-nutritive sinapoylcholine (sinapine) in the seed. We successfully down-regulated activities of key enzymes in the pathway including F5H and SCT and achieved reduction of sinapine and lignin in B. napus seeds. Despite this success, it was unclear how multiple agronomic traits were affected in the transgenic plants. Here, we report altered large-scale gene expression of new alleles of f5h and sct mutants of A. thaliana and resultant accumulation of sinapoylglucose, disinapoylglucose, quercetin-3-O-rhamnoside, salicylic acid glucoside, and total indolyl glucosinolates in the two mutants. Expression of several flowering genes was altered in these mutants when grown under drought and NaCl treatments. Furthermore, both mutants were more susceptible to fungal infection than the wild type. Microarray experiments identified distinctive spatial and temporal expression patterns of gene clusters involved in silique/seed developmental processes and metabolite biosynthesis in these mutants. Taken together, these findings suggest that both f5h and sct mutants exhibit major differences in accumulation of diverse metabolites in the seed and profound changes in global large-scale gene expression, resulting in differential pleiotropic responses to environmental cues. Electronic supplementary material The online version of this article (doi:10.1007/s00425-009-1007-2) contains supplementary material, which is available to authorized users.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.