ArticlePLoS genetics2009
Regulation of heterochromatin assembly on unpaired chromosomes during Caenorhabditis elegans meiosis by components of a small RNA-mediated pathway.
Article in PLoS genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 89 citations in OpenAlex.
- SIN-3 acts in distinct complexes to regulate the germline transcriptional program in Caenorhabditis elegans.Development (Cambridge, England) · 2023Article
- A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes.The EMBO journal · 2023Article
- Widespread transposon co-option in theScience advances · 2022Article
- TRIM-NHL protein, NHL-2, modulates cell fate choices in the C. elegans germ line.Developmental biology · 2022Article
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- Two isoforms of the essential C. elegans Argonaute CSR-1 differentially regulate sperm and oocyte fertility.Nucleic acids research · 2021Article
- Arginine methylation promotes siRNA-binding specificity for a spermatogenesis-specific isoform of the Argonaute protein CSR-1.Nature communications · 2021Article
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- Meiotic H3K9me2 distribution is influenced by the ALG-3 and ALG-4 pathway and by poly(U) polymerase activity.microPublication biology · 2021Article
- Meiotic Double-Strand Break Processing and Crossover Patterning Are Regulated in a Sex-Specific Manner by BRCA1-BARD1 inGenetics · 2020Article
- Conserved chromosomal functions of RNA interference.Nature reviews. Genetics · 2020Review
- Multigenerational Regulation of theAnnual review of genetics · 2019Review
- Biology of theGenetics · 2019Review
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- The balance of poly(U) polymerase activity ensures germline identity, survival and development inDevelopment (Cambridge, England) · 2018Article
- Functional Interactions BetweenG3 (Bethesda, Md.) · 2018Article
- The Molecular Chaperone HSP90 Promotes Notch Signaling in the Germline ofG3 (Bethesda, Md.) · 2018Article
- The CSR-1 endogenous RNAi pathway ensures accurate transcriptional reprogramming during the oocyte-to-embryo transition in Caenorhabditis elegans.PLoS genetics · 2018Article
- Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Many organisms have a mechanism for down regulating the expression of non-synapsed chromosomes and chromosomal regions during meiosis. This phenomenon is thought to function in genome defense. During early meiosis in Caenorhabditis elegans, unpaired chromosomes (e.g., the male X chromosome) become enriched for a modification associated with heterochromatin and transcriptional repression, dimethylation of histone H3 on lysine 9 (H3K9me2). This enrichment requires activity of the cellular RNA-directed RNA polymerase, EGO-1. Here we use genetic mutation, RNA interference, immunofluorescence microscopy, fluorescence in situ hybridization, and molecular cloning methods to identify and analyze three additional regulators of meiotic H3K9me2 distribution: CSR-1 (a Piwi/PAZ/Argonaute protein), EKL-1 (a Tudor domain protein), and DRH-3 (a DEAH/D-box helicase). In csr-1, ekl-1, and drh-3 mutant males, we observed a reduction in H3K9me2 accumulation on the unpaired X chromosome and an increase in H3K9me2 accumulation on paired autosomes relative to controls. We observed a similar shift in H3K9me2 pattern in hermaphrodites that carry unpaired chromosomes. Based on several assays, we conclude that ectopic H3K9me2 accumulates on paired and synapsed chromosomes in these mutants. We propose alternative models for how a small RNA-mediated pathway may regulate H3K9me2 accumulation during meiosis. We also describe the germline phenotypes of csr-1, ekl-1, and drh-3 mutants. Our genetic data suggest that these factors, together with EGO-1, participate in a regulatory network to promote diverse aspects of development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.